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Flow Cytometry: Difference between revisions

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<span style="color:navy>''CD-classified markers frequently used in leukaemia diagnosis''
<span style="color:navy>''CD-classified markers frequently used in leukaemia diagnosis''


{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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'''[[CD1A|<span style="color:#0b7514">'''CD1A'''</span>]]'''
'''[[CD1A|<span style="color:#0b7514">'''CD1A'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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'''[[CD2|<span style="color:#0b7514">'''CD2'''</span>]]'''
'''[[CD2|<span style="color:#0b7514">'''CD2'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD3|<span style="color:#0b7514">''CD3''</span>]]'''
'''[[CD3|<span style="color:#0b7514">''CD3''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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|
'''[[CD4 & CD8|<span style="color:#0b7514">''CD4''</span>]]'''
'''[[CD4 & CD8|<span style="color:#0b7514">''CD4''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD5|<span style="color:#0b7514">''CD5''</span>]]'''
'''[[CD5|<span style="color:#0b7514">''CD5''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD7|<span style="color:#0b7514">''CD7''</span>]]'''
'''[[CD7|<span style="color:#0b7514">''CD7''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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'''[[CD4 & CD8|<span style="color:#0b7514">''CD8''</span>]]'''
'''[[CD4 & CD8|<span style="color:#0b7514">''CD8''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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|
'''[[CD10|<span style="color:#0b7514">'''CD10'''</span>]]'''
'''[[CD10|<span style="color:#0b7514">'''CD10'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD11b|<span style="color:#0b7514">'''CD11b'''</span>]]'''
'''[[CD11b|<span style="color:#0b7514">'''CD11b'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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'''[[CD11c|<span style="color:#0b7514">'''CD11c'''</span>]]'''
'''[[CD11c|<span style="color:#0b7514">'''CD11c'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD13|<span style="color:#0b7514">''CD13''</span>]]'''
'''[[CD13|<span style="color:#0b7514">''CD13''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
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'''[[CD14|'''<span style="color:#0b7514">''CD14''</span>]]'''
'''[[CD14|'''<span style="color:#0b7514">''CD14''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD15|<span style="color:#0b7514">'''CD15'''</span>]]'''
'''[[CD15|<span style="color:#0b7514">'''CD15'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD16|<span style="color:#0b7514">'''CD16'''</span>]]'''
'''[[CD16|<span style="color:#0b7514">'''CD16'''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD19|'''<span style="color:#0b7514">''CD19''</span>]]'''
'''[[CD19|'''<span style="color:#0b7514">''CD19''</span>]]'''
|}  
|}  
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
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'''[[CD20|'''<span style="color:#0b7514">''CD20''</span>]]'''
'''[[CD20|'''<span style="color:#0b7514">''CD20''</span>]]'''
|}  
|}  
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD22|'''<span style="color:#0b7514">''CD22''</span>]]'''
'''[[CD22|'''<span style="color:#0b7514">''CD22''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD23|'''<span style="color:#0b7514">''CD23''</span>]]'''
'''[[CD23|'''<span style="color:#0b7514">''CD23''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD24|'''<span style="color:#0b7514">''CD24''</span>]]'''
'''[[CD24|'''<span style="color:#0b7514">''CD24''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD25|'''<span style="color:#0b7514">''CD25''</span>]]'''
'''[[CD25|'''<span style="color:#0b7514">''CD25''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD33|'''<span style="color:#0b7514">''CD33''</span>]]'''
'''[[CD33|'''<span style="color:#0b7514">''CD33''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD34|'''<span style="color:#0b7514">''CD34''</span>]]'''
'''[[CD34|'''<span style="color:#0b7514">''CD34''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD38|'''<span style="color:#0b7514">''CD38''</span>]]'''
'''[[CD38|'''<span style="color:#0b7514">''CD38''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD41|'''<span style="color:#0b7514">''CD41''</span>]]'''
'''[[CD41|'''<span style="color:#0b7514">''CD41''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
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'''[[CD43|'''<span style="color:#0b7514">''CD43''</span>]]'''
'''[[CD43|'''<span style="color:#0b7514">''CD43''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD56|'''<span style="color:#0b7514">''CD56''</span>]]'''
'''[[CD56|'''<span style="color:#0b7514">''CD56''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
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'''[[CD57|'''<span style="color:#0b7514">''CD57''</span>]]'''
'''[[CD57|'''<span style="color:#0b7514">''CD57''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD61|'''<span style="color:#0b7514">''CD61''</span>]]'''
'''[[CD61|'''<span style="color:#0b7514">''CD61''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD64|'''<span style="color:#0b7514">''CD64''</span>]]'''
'''[[CD64|'''<span style="color:#0b7514">''CD64''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD65|'''<span style="color:#0b7514">''CD65''</span>]]'''
'''[[CD65|'''<span style="color:#0b7514">''CD65''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD79a|'''<span style="color:#0b7514">''CD79a''</span>]]'''
'''[[CD79a|'''<span style="color:#0b7514">''CD79a''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD103|'''<span style="color:#0b7514">''CD103''</span>]]'''
'''[[CD103|'''<span style="color:#0b7514">''CD103''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD117|'''<span style="color:#0b7514">''CD117''</span>]]'''
'''[[CD117|'''<span style="color:#0b7514">''CD117''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD123|'''<span style="color:#0b7514">''CD123''</span>]]'''
'''[[CD123|'''<span style="color:#0b7514">''CD123''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD138|'''<span style="color:#0b7514">''CD138''</span>]]'''
'''[[CD138|'''<span style="color:#0b7514">''CD138''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD200|'''<span style="color:#0b7514">''CD200''</span>]]'''
'''[[CD200|'''<span style="color:#0b7514">''CD200''</span>]]'''
|}
|}
{|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
{|style="float:left; width: 80px; height: 40px; text-align:center; font-style: bold; color: #0b7514; background: #e4f4e5; border-style: solid; border-width: 2px; margin: 5px"
|
|
'''[[CD235|'''<span style="color:#0b7514">''CD235''</span>]]'''
'''[[CD235|'''<span style="color:#0b7514">''CD235''</span>]]'''

Revision as of 20:39, 17 June 2023

  • Individual immunophenotypic markers used in diagnosis
  • Reactivity patterns used to distinguish disorders (reactivity patterns)
  • Immuonophenotype of specific conditions (disease specific patterns)


Markers used mainly for gating:

CD45


Non CD-classified markers frequently used in haematological diagnosis

kappa

lambda

FMC7

TDT

MPO

TCRαβ

TCRγδ

HLA-DR

IgM



CD-classified markers frequently used in leukaemia diagnosis

CD1A

CD2

CD3

CD4

CD5

CD7

CD8

CD10

CD11b

CD11c

CD13

CD14

CD15

CD16

CD19

CD20

CD22

CD23

CD24

CD25

CD33

CD34

CD38

CD41

CD43

CD56

CD57

CD61

CD64

CD65

CD79a

CD103

CD117

CD123

CD138

CD200

CD235






test

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