MPAL: Difference between revisions
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<span style="font-size:80%; text-align:left;"></br>For B-lymphoid lineage assignment the key lineage-marker is CD19. However, this marker has recognised aberrant expression in AML cases so additional criteria of expression intensity it is required that other markers must be expressed in addition to allow B-lineage assignment.</br> | <span style="font-size:80%; text-align:left;"></br>For B-lymphoid lineage assignment the key lineage-marker is CD19. However, this marker has recognised aberrant expression in AML cases so additional criteria of expression intensity it is required that other markers must be expressed in addition to allow B-lineage assignment.</br> | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |'''Marker combination 1''' | |||
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|colspan="1" style = "font-size:90%; color:black;"|'''Required''' [[CD19|Strong expression of CD19]] | |||
|colspan="1" style = "font-size:90%;"|To meet the definition of "strong" the expression intensity '''must exceed''' that of 50% of normal B lymphocytes | |||
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|colspan="1" style = "font-size:90%;"|'''In addition''': | |||
|colspan="1" style = "font-size:90%;"|There must be expression of '''at least ONE''' of the following [[CD10]], [[CD22]], or [[CD79a]] | |||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |'''Marker combination 2''' | |||
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|colspan="1" style = "font-size:90%; color:black;"|'''Required''' [[CD19|Weak expression of CD19]] | |||
|colspan="1" style = "font-size:90%;"|To meet the definition of "weak" the expression intensity must be '''less than''' that of 50% of normal B lymphocytes | |||
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|colspan="1" style = "font-size:90%;"|'''In addition''': | |||
|colspan="1" style = "font-size:90%;"|There must be additional expression of '''at least TWO''' of the following [[CD10]], [[CD22]], or [[CD79a]] | |||
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!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">|'''Lineage assignments 2 - defining B-lymphoid lineage in MPAL'''</br></span> | |||
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<span style="font-size:80%; text-align:left;"></br>For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br> | |||
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Revision as of 10:34, 27 July 2023
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Lineage assignments 1 - defining Myeloid lineage in MPAL
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Myeloid markers option 1 | |
Myeloperoxidase (MPO) | This marker alone is sufficient to establish myeloid lineage provided it meets the intensity definition - intensity should be at least half of that of mature neutrophils in at least of proportion of cells. |
Myeloid markers option 2 | |
If MPO does not meet criteria for myeloid lineage, then myeloid lineage may still be assigned through demonstration of monocytic lineage. This can be assigned by the detection of at least two of the following features: | |
Surface markers of monocytic lineage: CD11c, CD14, CD64 or other recognised features of monocytic differentiation: serum or urinary lysozyme or non-specific esterase (detected by enzyme cytochemistry) |
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Lineage assignments 2 - defining B-lymphoid lineage in MPAL
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Marker combination 1 | |
Required Strong expression of CD19 | To meet the definition of "strong" the expression intensity must exceed that of 50% of normal B lymphocytes |
In addition: | There must be expression of at least ONE of the following CD10, CD22, or CD79a |
Marker combination 2 | |
Required Weak expression of CD19 | To meet the definition of "weak" the expression intensity must be less than that of 50% of normal B lymphocytes |
In addition: | There must be additional expression of at least TWO of the following CD10, CD22, or CD79a |
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Lineage assignments 2 - defining B-lymphoid lineage in MPAL
| |
---|---|
Marker combination 1 | |
Required Strong expression of CD19 | To meet the definition of "strong" the expression intensity must exceed that of 50% of normal B lymphocytes |
In addition: | There must be expression of at least ONE of the following CD10, CD22, or CD79a |
Marker combination 2 | |
Required Weak expression of CD19 | To meet the definition of "weak" the expression intensity must be less than that of 50% of normal B lymphocytes |
In addition: | There must be additional expression of at least TWO of the following CD10, CD22, or CD79a |