Additional immunophenotypic markers useful in lineage assignment or subtyping of AML
From haematologyetc.co.uk
Table: Marker patterns that can contribute to diagnosis or subtyping in less common cases
Additional markers that may support granulocytic maturation | |
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Generally the standard lineage-defining or lineage-associated markers in AML will allow assignment of granulocytic cases, other markers may be less specific but occasionally useful. | |
Additional features of granulocytic maturation | |
CD11b | A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies |
point 2 | |
Addtional features of monocytic differentiation | |
CD11c | This marker is most associated with monocytic maturation in AML being fairly well corellated with CD11B, but overall probably less specific for monocytic differentiation that CD14 |
CD14 | Primarily a marker of monocytic maturation in AML, seen most often in more differentiated forms, when present CD14 be considered a strong indicator of monocytic phenotype. |
CD64 | A good lineage marker for monocytic differentiation in AML, expressed in both monoblastic and monocytic forms, not fully sepicific when expressed at lower levels. |
point 3 | |
Features associated with erythroid differentiation | |
Most often CD34, CD45 and HLA-DR are weak or negative, although CD117 and CD36 are generally expressed. There may be some expression of platelet markers in some cases. | |
CD71 | Frequently expressed though not fully lineage specific |
CD235 | A good marker of erythroid differentiation but acquired late and therefore may not be expressed |
point 4 | |
Features associated with megakaryocytic differentiation | |
Most often CD34, CD45 and HLA-DR are weak or negative, although CD13 and CD33 may be expressed | |
CD41 | Platelet glycoprotein IIbIIIa |
CD61 | Platelet glycoprotein IIIa |
CD36 | Relatively non-specific (seen in erythroid and monocytic leukaemias) but often strongly expressed
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