Additional immunophenotypic markers useful in lineage assignment or subtyping of AML: Difference between revisions
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'' | ''These markers are not considered to define myeloid lineage, and generally have lower lineage specificity than myeloid-lineage associated markers, however they can support classification or support lineage assignment in difficult cases''</br></br> | ||
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{| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | {| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | ||
!colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">| | !colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers that may be used to support granulocytic or monocytic maturation in AML'''</span><span style="font-size:90%; text-align:left; background:white"> | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" | | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |These may be less specific than other markers but can be a useful adjunct in difficult cases | ||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD11b]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD11b]]''' | ||
|colspan="1" style = "font-size:84%;"|A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies | |colspan="1" style = "font-size:84%;"|A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies | ||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD11c]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD11c]]''' | ||
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{| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | {| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | ||
!colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">| | !colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with erythroid differentiation in AML'''</span><span style="font-size:90%; text-align:left; background:white"> | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |Most often AML with erythroid maturation will express [[CD34]], while [[CD45]] and [[HLA-DR]] are weak or negative. [[CD117]] is generally expressed. There may be some expression of megkaryocytic markers in some cases. | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |Most often AML with erythroid maturation will express [[CD34]], while [[CD45]] and [[HLA-DR]] are weak or negative. [[CD117]] is generally expressed. There may be some expression of megkaryocytic markers in some cases. | ||
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{| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | {| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | ||
!colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">| | !colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with megakaryocytic differentiation in AML'''</span><span style="font-size:90%; text-align:left; background:white"> | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |Most often [[CD34]], [[CD45]] and [[HLA-DR]] are weak or negative, although [[CD13]] and [[CD33]] may be expressed | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |Most often [[CD34]], [[CD45]] and [[HLA-DR]] are weak or negative, although [[CD13]] and [[CD33]] may be expressed | ||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD36]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD36]]''' | ||
|colspan="1" style = "font-size:84%;"|Relatively non-specific (seen in erythroid and monocytic leukaemias) but often strongly expressed | |colspan="1" style = "font-size:84%;"|Relatively non-specific (seen in erythroid and monocytic leukaemias) but often strongly expressed | ||
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{| class="wikitable" style="border-style: solid; border-width: 5px; color:black" | |||
!colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with basophilic differentiation in AML'''</span><span style="font-size:90%; text-align:left; background:white"> | |||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |comment | |||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD22]]''' | |||
|colspan="1" style = "font-size:84%;"| | |||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD123]]''' | |||
|colspan="1" style = "font-size:84%;"| | |||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD203]]''' | |||
|colspan="1" style = "font-size:84%;"| | |||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD11b]]''' | |||
|colspan="1" style = "font-size:84%;"| | |||
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Latest revision as of 14:19, 12 January 2024
These markers are not considered to define myeloid lineage, and generally have lower lineage specificity than myeloid-lineage associated markers, however they can support classification or support lineage assignment in difficult cases
| Markers that may be used to support granulocytic or monocytic maturation in AML | |
|---|---|
| These may be less specific than other markers but can be a useful adjunct in difficult cases | |
| CD11b | A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies |
| CD11c | This marker is most associated with monocytic maturation in AML being fairly well corellated with CD11B, but overall probably less specific for monocytic differentiation that CD14
|
| Markers associated with erythroid differentiation in AML | |
|---|---|
| Most often AML with erythroid maturation will express CD34, while CD45 and HLA-DR are weak or negative. CD117 is generally expressed. There may be some expression of megkaryocytic markers in some cases. | |
| CD36 | Expression is expected, but often expressed in other subtypes so not fully lineage specific |
| CD71 | Frequently expressed though not fully lineage specific |
| CD235 | A good marker of erythroid differentiation but acquired late and therefore may not be expressed
|
| Markers associated with megakaryocytic differentiation in AML | |
|---|---|
| Most often CD34, CD45 and HLA-DR are weak or negative, although CD13 and CD33 may be expressed | |
| CD41 | Platelet glycoprotein IIbIIIa |
| CD61 | Platelet glycoprotein IIIa |
| CD36 | Relatively non-specific (seen in erythroid and monocytic leukaemias) but often strongly expressed
|