Additional immunophenotypic markers useful in lineage assignment or subtyping of AML: Difference between revisions
From haematologyetc.co.uk
No edit summary |
No edit summary |
||
Line 14: | Line 14: | ||
!colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:pale gray">|Myeloid lineage-associated markers (Specific differentiation)'''</span><span style="font-size:90%; text-align:left; background:white"> | !colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:pale gray">|Myeloid lineage-associated markers (Specific differentiation)'''</span><span style="font-size:90%; text-align:left; background:white"> | ||
|- | |- | ||
|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" | | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |point 1 | ||
|- | |- | ||
!colspan="2" style = "font-size:90%;background:white;"|Additional features of granulocytic maturation | !colspan="2" style = "font-size:90%;background:white;"|Additional features of granulocytic maturation | ||
Line 20: | Line 20: | ||
|colspan="1" style = "font-size:90%; color:black;" |'''[[CD11b]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD11b]]''' | ||
|colspan="1" style = "font-size:84%;"|A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies | |colspan="1" style = "font-size:84%;"|A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies | ||
|- | |||
|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |point 2 | |||
|- | |- | ||
!colspan="2" style = "font-size:90%;background:white;"|Addtional features of monocytic differentiation | !colspan="2" style = "font-size:90%;background:white;"|Addtional features of monocytic differentiation | ||
Line 31: | Line 33: | ||
|colspan="1" style = "font-size:90%; color:black;" |'''[[CD64]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD64]]''' | ||
|colspan="1" style = "font-size:84%;"|A good lineage marker for monocytic differentiation in AML, expressed in both monoblastic and monocytic forms, not fully sepicific when expressed at lower levels. | |colspan="1" style = "font-size:84%;"|A good lineage marker for monocytic differentiation in AML, expressed in both monoblastic and monocytic forms, not fully sepicific when expressed at lower levels. | ||
|- | |||
|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |point 3 | |||
|- | |- | ||
!colspan="2" style = "font-size:90%;background:white;"|Features associated with erythroid differentiation | !colspan="2" style = "font-size:90%;background:white;"|Features associated with erythroid differentiation | ||
Line 41: | Line 45: | ||
|colspan="1" style = "font-size:90%; color:black;" |'''[[CD235]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD235]]''' | ||
|colspan="1" style = "font-size:84%;"|A good marker of erythroid differentiation but acquired late and therefore may not be expressed | |colspan="1" style = "font-size:84%;"|A good marker of erythroid differentiation but acquired late and therefore may not be expressed | ||
|- | |||
|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |point 4 | |||
|- | |- | ||
!colspan="2" style = "font-size:90%;background:white;"|Features associated with megakaryocytic differentiation | !colspan="2" style = "font-size:90%;background:white;"|Features associated with megakaryocytic differentiation |
Revision as of 17:58, 20 December 2023
Table: Marker patterns that allow definite diagnosis of acute myeloid leukaemia
Myeloid lineage-associated markers (Specific differentiation) | |
---|---|
point 1 | |
Additional features of granulocytic maturation | |
CD11b | A marker of both granulocytic and monocytic maturation, this marker has previously been associated with less good outcome in a number of studies |
point 2 | |
Addtional features of monocytic differentiation | |
CD11c | This marker is most associated with monocytic maturation in AML being fairly well corellated with CD11B, but overall probably less specific for monocytic differentiation that CD14 |
CD14 | Primarily a marker of monocytic maturation in AML, seen most often in more differentiated forms, when present CD14 be considered a strong indicator of monocytic phenotype. |
CD64 | A good lineage marker for monocytic differentiation in AML, expressed in both monoblastic and monocytic forms, not fully sepicific when expressed at lower levels. |
point 3 | |
Features associated with erythroid differentiation | |
Most often CD34, CD45 and HLA-DR are weak or negative, although CD117 and CD36 are generally expressed. There may be some expression of platelet markers in some cases. | |
CD71 | Frequently expressed though not fully lineage specific |
CD235 | A good marker of erythroid differentiation but acquired late and therefore may not be expressed |
point 4 | |
Features associated with megakaryocytic differentiation | |
Most often CD34, CD45 and HLA-DR are weak or negative, although CD13 and CD33 may be expressed | |
CD41 | Platelet glycoprotein IIbIIIa |
CD61 | Platelet glycoprotein IIIa |
CD36 | Relatively non-specific (seen in erythroid and monocytic leukaemias) but often strongly expressed
|