Marker patterns erythroid or megakaryocyte differentiation: Difference between revisions
From haematologyetc.co.uk
(Created page with "---- <div style="width: 200px"> {| class="wikitable" style="border-left:solid 5px green;border-right:solid 5px green;border-top:solid 5px black;border-bottom:solid 5px black; font-size:90%; color:navy" | colspan="1"''|Return to previous page'' |} </div> ---- <span style="font-size:90%>'''Acute erythroid leukaemia (AEL)'''</br>This may be a difficult diagnosis since markers will often not allow cells to be distinguished from a re...") |
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!colspan="2" <span style="font-size:90%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with erythroid differentiation in | !colspan="2" <span style="font-size:90%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with erythroid differentiation in myeloid precursor neoplams'''</span><span style="font-size:90%; text-align:left; background:white"></span> | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |'''General:''' | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |'''General:''' General markers may vary, but typically patterns are given below: | ||
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|colspan="2" style = "font-size:90%; color:black;" |'''Specific:''' Markers of erythroid differentiation are helpful, but require careful interpretation to discriminate from other causes (described below). | |colspan="2" style = "font-size:90%; color:black;" |There is very weak expression of [[CD45]] and [[HLA-DR]]. In contrast [[CD34]] and often [[CD117]] will be detected. | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |'''Specific:''' Markers of erythroid differentiation are helpful, but require careful interpretation to discriminate from other causes (described below). | |||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD36]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD36]]''' | ||
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|colspan="1" style = "font-size:84%;"|A good marker of erythroid differentiation; however like CD72 does not distinguish neoplastic and reactive cells. It is acquired later in erythroid differentiation and therefore may not be expressed or be confined to a sub-population in AEL. | |colspan="1" style = "font-size:84%;"|A good marker of erythroid differentiation; however like CD72 does not distinguish neoplastic and reactive cells. It is acquired later in erythroid differentiation and therefore may not be expressed or be confined to a sub-population in AEL. | ||
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|colspan="2" style = "font-size:90%; color:black;" |'''Abberant | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |'''Abberant phenotypic features:''' Some markers are frequently found: | ||
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|colspan="2" style = "font-size:90%; color:black;" |Expression of [[CD13]], [[CD38]] or [[CD4]] may be encountered in some cases. | |||
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!colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with megakaryocytic differentiation in AML'''</span><span style="font-size:90%; text-align:left; background:white"> | !colspan="2" <span style="font-size:100%; colour:white; text-align:left; border: 1px solid black; background:lightgrey">|'''Markers associated with megakaryocytic differentiation in AML'''</span><span style="font-size:90%; text-align:left; background:white"> | ||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" | | |colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |This can be tricky initially since [[CD34]], [[CD45]] and [[HLA-DR]] are most often very weak or negative. [[CD13]] and [[CD33]] may be expressed but are not always present. Look for: | ||
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|colspan="1" style = "font-size:90%; color:black;" |'''[[CD41]]''' | |colspan="1" style = "font-size:90%; color:black;" |'''[[CD41]]''' | ||
Latest revision as of 11:43, 2 February 2024
Acute erythroid leukaemia (AEL)
This may be a difficult diagnosis since markers will often not allow cells to be distinguished from a reactive erythroid expansion, and the overlap with cases of myelodysplasia-related AML may have a marked erythroid expansion. In such cases the diagnosis of AEL requires careful exclusion of other disorders by correlation with morphology and other tests.
| Markers associated with erythroid differentiation in myeloid precursor neoplams | |
|---|---|
| General: General markers may vary, but typically patterns are given below: | |
| There is very weak expression of CD45 and HLA-DR. In contrast CD34 and often CD117 will be detected. | |
| Specific: Markers of erythroid differentiation are helpful, but require careful interpretation to discriminate from other causes (described below). | |
| CD36 | Expression is expected, but is not fully lineage specific as it may be seen in other AML forms including cases with monocytic or megakaryocytic differentiation. |
| CD71 | A good marker of early erythroid differentiation that is expected to be expressed, although again not fully lineage specific and also found on reactive erythroid precursors. |
| CD235 | A good marker of erythroid differentiation; however like CD72 does not distinguish neoplastic and reactive cells. It is acquired later in erythroid differentiation and therefore may not be expressed or be confined to a sub-population in AEL. |
| Abberant phenotypic features: Some markers are frequently found: | |
| Expression of CD13, CD38 or CD4 may be encountered in some cases.
| |
| Markers associated with megakaryocytic differentiation in AML | |
|---|---|
| This can be tricky initially since CD34, CD45 and HLA-DR are most often very weak or negative. CD13 and CD33 may be expressed but are not always present. Look for: | |
| CD41 | Platelet glycoprotein IIbIIIa |
| CD61 | Platelet glycoprotein IIIa |
| CD36 | Relatively non-specific (seen in erythroid and monocytic leukaemias) but often strongly expressed
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