The flow cytometric diagnosis of AML
From haematologyetc.co.uk
1. Do the cells have flow cytometric features that support their primitive nature?
- Most often in AML useful features of immature cells are weak expression of CD45, and expression of CD34 and/or CD117. However, a wider panel may be considered (for more detail see table)
- In some cases there may be atypical features where primitive nature is less clear, this generally concerns some monocytic cases or APL (see table for details)
Can we confirm myeloid nature?
- Typical cases can generally be recognised by (see table for details)
- Atypical cases can still be identified based on a minimal feature set (see table for details)
- In unusual cases an extended marker panel may allow lineage assignment (see Table for details)
Are there atypical features?
- Expected abberency
- Feature that may give concern