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<div style="background-color: #E0EEEE; {{round corners}} padding: 2em 2em; margin-right: 5px;"> | |||
:CD3 is a reliable marker of T cell lineage that is primarily used to distinguish between T and B cells. The pattern of expression has diagnostic relevance. | |||
<span style="color:navy> | </div> | ||
<span style="color:navy">'''Normal expression and function'''</span> | |||
CD3 is a multi-molecular complex that associates with the T cell receptor of mature T cells, and supports TCR expression and intracellular signalling in response to antigen. It is first expressed in the cytoplasm of pro-thymocytes before migrating to the T-cell surface during thymic maturation. CD3 expression is almost exclusive to mature T cells (there is weak expression of CD3 by some macrophages). | |||
<span style="color:navy">'''Diagnostic role'''</span> | |||
* '''T-ALL''''' Expression is mainly cytoplasmic (cyCD3). Surface (sCD3) is usually absent in Early T-precursor T-ALL but is variably expressed in more mature T-ALL. | |||
* '''Mature T cell neoplasms''': sCD3 is expressed on T cell lymphoproliferative disorders. The level of CD3 expression is increased (compared to normal T cells) in T-PLL and T-LGL, and is decreased in Sézary syndrome and ATLL | |||
* '''Natural Killer cell lymphoproliferative disorders''': do not express a conventional TCR so CD3 is not expressed. | |||
* '''Other''' B cells rarely express CD3, and aberrant expression in AML is rare (in contrast to other T cell markers such as CD2 or CD7). | |||
---- | ---- | ||
<span style="color:navy">'''''SUMMARY TABLES'''''</span> | |||
Expression by acute leukaemias and by haematogones | |||
<gallery widths="40px" heights="40px" > | |||
File:rare.png|''Pro-B ALL''|link=Flow cytometry key | |||
File:rare.png|''Early pre-B ALL''|link=Flow cytometry key | |||
File:rare.png|''Pre-B ALL''|link=Flow cytometry key | |||
File:rare.png|''B ALL''|link=Flow cytometry key | |||
File:Hi.png|''Pro-T ALL''|link=Flow cytometry key | |||
File:Hi.png|''Mature-T ALL''|link=Flow cytometry key | |||
File:Poss.png|''AML'' *|link=Flow cytometry key | |||
File:Rare.png|''Haemato-gones''|link=Flow cytometry key | |||
</gallery> | |||
<span style="font-size:80%">'''*''' May be seen in AML but consider bi-phenotypic if present</span> | |||
Expression by B-lymphoproliferative disorders | |||
<gallery widths="40px" heights="40px" > | |||
File:rare.png|frame|''CLL''|link=Flow cytometry key | |||
File:rare.png|''PLL''|link=Flow cytometry key | |||
File:rare.png|''MCL''|link=Flow cytometry key | |||
File:Poss.png|''FL'' *|link=Flow cytometry key | |||
File:rare.png|''HCL''|link=Flow cytometry key | |||
File:rare.png|''HCLv''|link=Flow cytometry key | |||
File:rare.png|''MZL''|link=Flow cytometry key | |||
File:rare.png|''LPL''|link=Flow cytometry key | |||
File:rare.png|''PCs''|link=Flow cytometry key | |||
</gallery> | |||
<span style="font-size:80%">'''*''' occasionally described in FL</span> | |||
Expression by mature T-cell disorders | |||
< | <gallery widths="40px" heights="40px" > | ||
File:Hi.png|frame|''T-PLL''|link=Flow cytometry key | |||
File:Hi.png|''T-LGL''|link=Flow cytometry key | |||
File:Rare.png|''NK-LGL''|link=Flow cytometry key | |||
File:Hi.png|''ATLL'' *|link=Flow cytometry key | |||
File:Hi.png|''Sezary'' *|link=Flow cytometry key | |||
File:Freq.png|''Others'' **|link=Flow cytometry key | |||
</gallery> | |||
<span style="font-size:80%">'''*''' expression may be dim</span> | |||
<span style="font-size:80%">'''**''' a range of other T cell disorders may circulate occasionally, marker expression varies</span> | |||
---- | ---- | ||
'''KEY''' | |||
<div class="toccolours mw-collapsible mw-collapsed" style="font-size 80%; overflow:auto;"> | |||
The indicated expression frequency, indicates the approximate frequency of occurrence in each particular disease type. | |||
[[File:Rare.png|30px|link=]] | |||
--- | rare (<5%) | ||
[[File:Poss.png|30px|link=]] | |||
possible (5-20%) | |||
[[File:Mod.png|30px|link=]] | |||
likely (20-40%) | |||
[[File:Freq.png|30px|link=]] | |||
frequent (40-80%) | |||
[[File:Hi.png|30px|link=]] | |||
expected (>80%) | |||
Where expression level is unusual or characteristic of a disorder this is indicated by text: | |||
</div> | |||
---- | ---- |
Latest revision as of 13:17, 3 March 2023
- CD3 is a reliable marker of T cell lineage that is primarily used to distinguish between T and B cells. The pattern of expression has diagnostic relevance.
Normal expression and function
CD3 is a multi-molecular complex that associates with the T cell receptor of mature T cells, and supports TCR expression and intracellular signalling in response to antigen. It is first expressed in the cytoplasm of pro-thymocytes before migrating to the T-cell surface during thymic maturation. CD3 expression is almost exclusive to mature T cells (there is weak expression of CD3 by some macrophages).
Diagnostic role
- T-ALL Expression is mainly cytoplasmic (cyCD3). Surface (sCD3) is usually absent in Early T-precursor T-ALL but is variably expressed in more mature T-ALL.
- Mature T cell neoplasms: sCD3 is expressed on T cell lymphoproliferative disorders. The level of CD3 expression is increased (compared to normal T cells) in T-PLL and T-LGL, and is decreased in Sézary syndrome and ATLL
- Natural Killer cell lymphoproliferative disorders: do not express a conventional TCR so CD3 is not expressed.
- Other B cells rarely express CD3, and aberrant expression in AML is rare (in contrast to other T cell markers such as CD2 or CD7).
SUMMARY TABLES
Expression by acute leukaemias and by haematogones
- Rare.png
Pro-B ALL
- Rare.png
Early pre-B ALL
- Rare.png
Pre-B ALL
- Rare.png
B ALL
- Hi.png
Pro-T ALL
- Hi.png
Mature-T ALL
- Poss.png
AML *
- Rare.png
Haemato-gones
* May be seen in AML but consider bi-phenotypic if present
Expression by B-lymphoproliferative disorders
- Rare.png
CLL
- Rare.png
PLL
- Rare.png
MCL
- Poss.png
FL *
- Rare.png
HCL
- Rare.png
HCLv
- Rare.png
MZL
- Rare.png
LPL
- Rare.png
PCs
* occasionally described in FL
Expression by mature T-cell disorders
- Hi.png
T-PLL
- Hi.png
T-LGL
- Rare.png
NK-LGL
- Hi.png
ATLL *
- Hi.png
Sezary *
- Freq.png
Others **
* expression may be dim
** a range of other T cell disorders may circulate occasionally, marker expression varies
KEY
The indicated expression frequency, indicates the approximate frequency of occurrence in each particular disease type.
rare (<5%)
possible (5-20%)
likely (20-40%)
frequent (40-80%)
expected (>80%)
Where expression level is unusual or characteristic of a disorder this is indicated by text: