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Background to flow cytometric diagnosis

Differentiation to plasma cells involves (in mast cases) the loss of typical pan B-cell markers and surface light chains. The expression of CD45 also differs both within and between cases making standard gating strategies difficult to apply. Cells are most often identified through their co-expression of CD138 with a relatively high expression of CD38, together with the absence of typical B-cell features.

Immunophenotype of plasma cells
CD45 expression
Typically expression of CD45 is similar to that of normal T and B cells.
Major markers useful in LPL diagnosis
Marker	Freq	Level	Comment
CD19		-	Generally CD19 will be expressed
CD38		-
κ/λ			Light chain retriction usually detected
CD5		-	Uncommon but seen in some cases
CD138		var	Particularly on cells with plasma cell features
CD200		-	Detected infrequently
FMC7			Generally expressed
Other relevant markers
CD10		-	Expression may be seen
CD11C		-
CD20		-	Expect expression
CD23		-	Infrequent but reported
CD25		-
CD43		-	Generally not expressed by LPL
CD79b		-	Generally expressed
CD103		-

Key to table:

Key to colour code for expression frequency Click for link

Key to expression strength code and use Click for link

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