Test
From haematologyetc.co.uk
Background
Typical CLL can be diagnosed with some confidence using flow cytometry. However, atypical cases are regularly encountered and can cause diagnostic difficulty.
Immunophenotype of CLL | |||
---|---|---|---|
Major markers useful in CLL diagnosis | |||
Marker | Freq | Level | Comment |
κ/λ | wk/ abs | Expect weak or absent restricted κ or λ expression | |
FMC7 | Wk | May be expressed by atypical cases, generally absent | |
CD5 | Mod | Characteristic of CLL, usually less strong than on T cells | |
CD10 | - | Rare in CLL, consider FL if detected | |
CD11C | Wk | Expressed in some cases, but tends to be weak | |
CD19 | Wk/ mod | Expression is expected, but less strong than normal B cells | |
CD20 | Wk | Expression is expected, but characteristically weak | |
CD23 | Wk/ Mod | Characteristic of CLL, although can rarely be weak or absent | |
CD25 | Wk/ Mod | Expressed by many cases of CLL but characteristically weak | |
CD38 | Wk | Expressed by some cases of CLL, typically weak | |
CD43 | Wk/ Mod | Similar to CD200, may be helpful in some cases | |
CD79a | Wk | Expression is expected, but characteristically weak | |
CD103 | - | Not expressed by CLL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation | |
CD200 | Mod | Characteristically positive in CLL and negative in MCL |
Key to table:
Click for key to expression frequency Key Click for key to expression strength Key