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|colspan="1" style = "font-size:90%;" |CD138 | |colspan="1" style = "font-size:90%;" |CD138 | ||
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|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|Particularly on cells with plasma cell features | ||
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Revision as of 10:49, 14 July 2023
Background to flow cytometric diagnosis
Cells of LPL share features of mature B-cell differentation and features of early plasma cell differentiation, and have mixed populations with features of each (although proportions vary). This can be a challenging diagnosis by flow cytometry alone, but other modalities can generally make this diagnosis clear.
Immunophenotype of LPL | |||
---|---|---|---|
CD45 expression | |||
Typically expression of CD45 is similar to that of normal T and B cells. | |||
Major markers useful in CLL diagnosis | |||
Marker | Freq | Level | Comment |
CD19 | - | Generally CD19 will be expressed | |
κ/λ | Light chain retriction usually detected | ||
CD5 | - | Uncommon but seen in some cases | |
CD23 | - | Infrequent but reported | |
CD79b | - | Generally expressed | |
CD200 | - | Detected infrequently | |
FMC7 | Generally expressed | ||
Other relevant markers | |||
CD10 | - | Expression may be seen | |
CD11C | - | ||
CD20 | - | Expect expression | |
CD25 | - | ||
CD38 | - | ||
CD43 | - | Generally not expressed by LPL | |
CD103 | - | ||
CD138 | - | Particularly on cells with plasma cell features |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link