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!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | !colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | ||
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|colspan="4" style = "font-size:90%; color:black;" | | |colspan="4" style = "font-size:90%; color:black;" |There is no consistent pattern of expression of CD45 although it is most often weak, but may be variable and is occasionally strong. | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in | !colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in plasma cell identification | ||
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|colspan="1" style = "font-size:80%; width: 4%; |'''Marker''' | |colspan="1" style = "font-size:80%; width: 4%; |'''Marker''' | ||
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|colspan="1" style = "background:#006699;"| | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|Expression of CD19 may persist in some cases | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD38 | |colspan="1" style = "font-size:90%; color:black;" |CD38 | ||
|colspan="1" style = "background:#006699;"| | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|The relatively strong expression of CD38 is typical of plasma cells | ||
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|colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ | |colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ | ||
|colspan="1" style = "width: 8%; background:#006699;"| | |colspan="1" style = "width: 8%; background:#006699;"| | ||
|colspan="1" style = "font-size:80%; width: 8%; | | |colspan="1" style = "font-size:80%; width: 8%; | | ||
|colspan="1" style = "font-size:80%;"| | |colspan="1" style = "font-size:80%;"|Surface light chain expression is lost (internal will be detected in permeabilised cells) | ||
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|colspan="1" style = "font-size:90%;" |CD138 | |colspan="1" style = "font-size:90%;" |CD138 | ||
|colspan="1" style = "background:#006699;"| | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|var | |colspan="1" style = "font-size:80%;"|var | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|Expression is expected although the intensity can vary | ||
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|colspan="1" style = "font-size:90%; color:black;" |FMC7 | |colspan="1" style = "font-size:90%; color:black;" |FMC7 | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers | !colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD5 | |||
|colspan="1" style = "background:#66e0ff;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:80%;"|Uncommon but seen in some cases | |||
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|colspan="1" style = "font-size:90%;" |CD10 | |colspan="1" style = "font-size:90%;" |CD10 | ||
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|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"| | ||
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|} | |colspan="1" style = "font-size:90%; color:black;" |CD200 | ||
|colspan="1" style = "background:#66e0ff;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:84%;"|Detected infrequently|-|} | |||
</div> | </div> | ||
Revision as of 16:17, 14 July 2023
Background to flow cytometric diagnosis
Differentiation to plasma cells involves (in mast cases) the loss of typical pan B-cell markers and surface light chains. The expression of CD45 also differs both within and between cases making standard gating strategies difficult to apply. Cells are most often identified through their co-expression of CD138 with a relatively high expression of CD38, together with the absence of typical B-cell features.
| Immunophenotype of plasma cells | |||
|---|---|---|---|
| CD45 expression | |||
| There is no consistent pattern of expression of CD45 although it is most often weak, but may be variable and is occasionally strong. | |||
| Major markers useful in plasma cell identification | |||
| Marker | Freq | Level | Comment |
| CD19 | - | Expression of CD19 may persist in some cases | |
| CD38 | - | The relatively strong expression of CD38 is typical of plasma cells | |
| κ/λ | Surface light chain expression is lost (internal will be detected in permeabilised cells) | ||
| CD138 | var | Expression is expected although the intensity can vary | |
| FMC7 | Generally expressed | ||
| Other relevant markers | |||
| CD5 | - | Uncommon but seen in some cases | |
| CD10 | - | Expression may be seen | |
| CD11C | - | ||
| CD20 | - | Expect expression | |
| CD23 | - | Infrequent but reported | |
| CD25 | - | ||
| CD43 | - | Generally not expressed by LPL | |
| CD79b | - | Generally expressed | |
| CD103 | - | ||
| CD200 | - | Detected infrequently|-|}
Key to table: Key to colour code for expression frequency Click for link Key to expression strength code and use Click for link
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