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<span style="color: navy>'''Background to flow cytometric diagnosis'''</span> | <span style="color: navy>'''Background to flow cytometric diagnosis'''</span> | ||
Differentiation to plasma cells involves (in mast cases) the loss of typical pan B-cell markers and surface light chains. The expression of CD45 also differs both within and between cases making standard gating strategies difficult to apply. Cells are most often identified through their co-expression of CD138 with a relatively high expression of CD38, together with the absence of typical B-cell features. | |||
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!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of | !colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of plasma cells''' | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | !colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression |
Revision as of 16:12, 14 July 2023
Background to flow cytometric diagnosis
Differentiation to plasma cells involves (in mast cases) the loss of typical pan B-cell markers and surface light chains. The expression of CD45 also differs both within and between cases making standard gating strategies difficult to apply. Cells are most often identified through their co-expression of CD138 with a relatively high expression of CD38, together with the absence of typical B-cell features.
Immunophenotype of plasma cells | |||
---|---|---|---|
CD45 expression | |||
Typically expression of CD45 is similar to that of normal T and B cells. | |||
Major markers useful in LPL diagnosis | |||
Marker | Freq | Level | Comment |
CD19 | - | Generally CD19 will be expressed | |
CD38 | - | ||
κ/λ | Light chain retriction usually detected | ||
CD5 | - | Uncommon but seen in some cases | |
CD138 | var | Particularly on cells with plasma cell features | |
CD200 | - | Detected infrequently | |
FMC7 | Generally expressed | ||
Other relevant markers | |||
CD10 | - | Expression may be seen | |
CD11C | - | ||
CD20 | - | Expect expression | |
CD23 | - | Infrequent but reported | |
CD25 | - | ||
CD43 | - | Generally not expressed by LPL | |
CD79b | - | Generally expressed | |
CD103 | - |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link