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[[file: | [[file:DLBCL.jpg|200px|link=BLBCL.jpg]] | ||
<span style="color: navy>'''Background to flow cytometric diagnosis'''</span> | <span style="color: navy>'''Background to flow cytometric diagnosis'''</span> | ||
This is an uncommon diagnosis by flow cytometry as DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease (ofetn when a diagnosis has already been made). However, occasionally these cases can present as a new diagnosis by blood or marrow and can be difficult as marker expression is often variable between cases. | |||
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|colspan="1" style = "font-size:90%; color:black;" |'''CD19''' | |colspan="1" style = "font-size:90%; color:black;" |'''CD19''' | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"| | ||
|- | |- | ||
|colspan="1" style = "width: 4%; font-size:90%; color:black;" |'''κ/λ''' | |colspan="1" style = "width: 4%; font-size:90%; color:black;" |'''κ/λ''' | ||
|colspan="1" style = "width: 8%; background:# | |colspan="1" style = "width: 8%; background:#006699;"| | ||
|colspan="1" style = "font-size:80%; width: 8%; | | |colspan="1" style = "font-size:80%; width: 8%; | | ||
|colspan="1" style = "font-size:80%;"|Expect weak restricted κ or λ or absent expression | |colspan="1" style = "font-size:80%;"|Expect weak restricted κ or λ or absent expression | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD25 | |colspan="1" style = "font-size:90%; color:black;" |CD25 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#66e0ff;"| | ||
|colspan="1" style = "font-size:80%;"|wk | |colspan="1" style = "font-size:80%;"|wk | ||
|colspan="1" style = "font-size:84%;"|Expressed by many cases of CLL but characteristically weak | |colspan="1" style = "font-size:84%;"|Expressed by many cases of CLL but characteristically weak | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD38 | |colspan="1" style = "font-size:90%; color:black;" |CD38 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#004466;"| | ||
|colspan="1" style = "font-size:80%;"|wk | |colspan="1" style = "font-size:80%;"|wk | ||
|colspan="1" style = "font-size:84%;"|Expressed by some cases of CLL, typically weak | |colspan="1" style = "font-size:84%;"|Expressed by some cases of CLL, typically weak | ||
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|colspan="1" style = "font-size:90%;" |CD43 | |colspan="1" style = "font-size:90%;" |CD43 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#66e0ff;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Similar to CD200, may be helpful in some cases | |colspan="1" style = "font-size:84%;"|Similar to CD200, may be helpful in some cases | ||
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|colspan="1" style = "font-size:90%;" |CD138 | |colspan="1" style = "font-size:90%;" |CD138 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#66e0ff;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation | |colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation |
Revision as of 12:54, 12 July 2023
Background to flow cytometric diagnosis
This is an uncommon diagnosis by flow cytometry as DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease (ofetn when a diagnosis has already been made). However, occasionally these cases can present as a new diagnosis by blood or marrow and can be difficult as marker expression is often variable between cases.
Immunophenotype of CLL | |||
---|---|---|---|
Major markers useful in CLL diagnosis | |||
Marker | Freq | Level | Comment |
CD19 | - | ||
κ/λ | Expect weak restricted κ or λ or absent expression | ||
CD5 | - | Characteristic of CLL, usually less strong than on T cells | |
CD23 | - | Characteristic of CLL, although can rarely be weak or absent | |
CD79b | wk | Expression is expected, but characteristically weak | |
CD200 | - | Characteristically positive in CLL and negative in MCL | |
FMC7 | May be expressed by atypical cases, generally absent | ||
Other relevant markers | |||
CD10 | - | Infrequent in CLL, consider FL if detected | |
CD11C | wk | Expressed in some cases, but tends to be weak | |
CD20 | wk | Expression is expected, but characteristically weak | |
CD25 | wk | Expressed by many cases of CLL but characteristically weak | |
CD38 | wk | Expressed by some cases of CLL, typically weak | |
CD43 | - | Similar to CD200, may be helpful in some cases | |
CD103 | - | Not expressed by CLL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link