Test: Difference between revisions
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|colspan="1" style = "font-size:90%; color:navy;" |<u>'''FMC7'''</u> | |colspan="1" style = "font-size:90%; color:navy;" |<u>'''FMC7'''</u> | ||
|colspan="1" style = "background:#006699;"| | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:80%;"|FMC7 is frequently expressed by typical MCL | |colspan="1" style = "font-size:80%;"|FMC7 is frequently expressed by typical MCL | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD25 | |colspan="1" style = "font-size:90%; color:black;" |CD25 | ||
|colspan="1" style = "background:#00b8e6;"| | |colspan="1" style = "background:#00b8e6;"| | ||
|colspan="1" style = "font-size:80%;"| | |colspan="1" style = "font-size:80%;"| | ||
|colspan="1" style = "font-size:84%;"|Not typical of MCL, not generally strong | |colspan="1" style = "font-size:84%;"|Not typical of MCL, not generally strong as HCL | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD38 | |colspan="1" style = "font-size:90%; color:black;" |CD38 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#004466;"| | ||
|colspan="1" style = "font-size:80%;"|wk | |colspan="1" style = "font-size:80%;"|wk | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|Expression is expected in MCL but CD138 should be absent | ||
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|colspan="1" style = "font-size:90%;" |CD43 | |colspan="1" style = "font-size:90%;" |CD43 |
Revision as of 22:50, 19 June 2023
Background
Typical CLL can be diagnosed with some confidence using flow cytometry.
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly in distinguishing CLL from atypical cases of MCL or MZL. If this is the case then the atypical features should be reported, and the possibility of alternative diagnoses acknowledged.
Note also that cases with a low lymphocytosis (<1x109) may represent monoclonal B-lymphocytosis with CLL phenotype (click here for more).
Immunophenotype of MCL | |||
---|---|---|---|
Major markers useful in CLL diagnosis | |||
Marker | Freq | Level | Comment |
κ/λ | Expect moderate or strong restricted κ or λ expression | ||
FMC7 | - | FMC7 is frequently expressed by typical MCL | |
CD5 | - | Expression of CD5 is characteristic of MCL | |
CD10 | - | Infrequently expressed, expression may suggest FL | |
CD11C | Expressed in some cases of MCL but not typical | ||
CD19 | - | Expression is expected, typically more strongly than in CLL | |
CD20 | wk | Expression is expected, typically stronger than CLL | |
CD23 | - | Typically not expressed by MCL, may rarely be expressed | |
CD25 | Not typical of MCL, not generally strong as HCL | ||
CD38 | wk | Expression is expected in MCL but CD138 should be absent | |
CD43 | - | Similar to CD200, may be helpful in some cases | |
CD79a | wk | Expression is expected, but characteristically weak | |
CD103 | - | Not expressed by CLL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation | |
CD200 | - | Characteristically positive in CLL and negative in MCL |
Key to table:
Markers most useful in initial diagnosis are underlined in blue text
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link