MPAL
From haematologyetc.co.uk
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Definition of MPAL (WHO)
Section 1 - defining Myeloid lineage in MPAL
(1) Typically AML blasts have low CD45 expression and cause low side scatter. This means that they form a relatively uniform and distinctive population that is clearly separate from that of lymphocytes on CD45/SSc plots (for further details see this section). It is important to realise however also not all AML cases may fit this pattern low CD45/low SSc pattern - particularly APL and monocytic AML. | |||
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Marker combination 1 | |||
Myeloperoxidase (MPO) | This marker alone is sufficient to establish myeloid lineage provided it meets the intensity definition - intensity should be at least half of that of mature neutrophils in at least of proportion of cells. | ||
Marker combination 2 | |||
If MPO does not meet criteria for myeloid lineage, then myeloid lineage may still be assigned through demonstration of monocytic lineage: AT LEAST TWO of the following markers are expressed: CD11c, CD14, CD64 | Alternatively moncytic differentiation may be established through detection of serum or urinary lysozyme; or through cytochemical detection of non-specific esterase |