MPAL: Difference between revisions
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Revision as of 19:19, 26 July 2023
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Lineage assignments 1 - defining Myeloid lineage in MPAL
(1) Typically AML blasts have low CD45 expression and cause low side scatter. This means that they form a relatively uniform and distinctive population that is clearly separate from that of lymphocytes on CD45/SSc plots (for further details see this section). It is important to realise however also not all AML cases may fit this pattern low CD45/low SSc pattern - particularly APL and monocytic AML. | |
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Marker combination 1 | |
Myeloperoxidase (MPO) | This marker alone is sufficient to establish myeloid lineage provided it meets the intensity definition - intensity should be at least half of that of mature neutrophils in at least of proportion of cells. |
Marker combination 2 | |
If MPO does not meet criteria for myeloid lineage, then myeloid lineage may still be assigned through demonstration of monocytic lineage. This can be assigned by the detection of AT LEAST TWO of the following | |
Surface markers of monocytic lineage: CD11c, CD14, CD64 or Other recognised features of monocytic differentiation: serum or urinary lysozyme or non-specific esterase (detected by enzyme cytochemistry) |
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Definition of MPAL (WHO)
Section 1 - defining Myeloid lineage in MPAL
(1) Typically AML blasts have low CD45 expression and cause low side scatter. This means that they form a relatively uniform and distinctive population that is clearly separate from that of lymphocytes on CD45/SSc plots (for further details see this section). It is important to realise however also not all AML cases may fit this pattern low CD45/low SSc pattern - particularly APL and monocytic AML. | |
---|---|
Marker combination 1 | |
Myeloperoxidase (MPO) | This marker alone is sufficient to establish myeloid lineage provided it meets the intensity definition - intensity should be at least half of that of mature neutrophils in at least of proportion of cells. |
Marker combination 2 | |
If MPO does not meet criteria for myeloid lineage, then myeloid lineage may still be assigned through demonstration of monocytic lineage. This can be assigned by the detection of AT LEAST TWO of the following | |
Surface markers of monocytic lineage: CD11c, CD14, CD64 or Other recognised features of monocytic differentiation: serum or urinary lysozyme or non-specific esterase (detected by enzyme cytochemistry) |