Flow cytometry: Follicular Lymphoma
From haematologyetc.co.uk
Typical FCL morphology
Background
Follicular lymphoma expresses CD10, with other immunophenotypic featues differing from related disorders.
However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing.
CD45 Expression
Side scatter
| Immunophenotype of FL | |||
|---|---|---|---|
| Major markers useful in CLL diagnosis | |||
| Marker | Freq | Level | Comment |
| κ/λ | Expect κ or λ expression, undetectable in rare cases | ||
| FMC7 | - | Occasionally seen, FMC7 is not typical of FL | |
| CD5 | - | Expression of CD5 is characteristic of MCL | |
| CD10 | Strg | Expression is expected though not universal in FL | |
| Other relevant markers | |||
| CD11C | Expression is not typically seen in FL | ||
| CD19 | - | Expression is expected, but may be weak or undectable | |
| CD20 | - | Expression is expected, rare cases may have CD20 loss | |
| CD23 | - | May be expressed by some cases of FL | |
| CD25 | Not typical of FL, where present it is not generally strong | ||
| CD38 | - | Expression is expected in FL but CD138 should be absent | |
| CD43 | - | Expression is uncommon in FL, may be helpful in some cases | |
| CD79b | Expression is expected and should be stronger than CLL | ||
| CD103 | - | Not expressed by FL, consider HCL if found | |
| CD138 | - | Predominantly expressed at plasma cell differentiation | |
| CD200 | - | Characteristically (though not always) negative in FL | |
Key to table:
Markers most useful in initial diagnosis are underlined in blue text
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link