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Flow cytometry: Follicular Lymphoma: Difference between revisions

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<gallery mode="nolines" widths="200px" heights="200px" border="1px" >
[[file:MCL.jpg|200px|link=MCL.jpg]] 
file:FL.jpg|link={{filepath:FL.jpg}}
 
</gallery>
<span style="font-size:90%">''Typical MCL morphology''</span>
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!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of FL'''
!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of FL'''
|-
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''Major markers useful in CLL diagnosis'''  
!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression
|-
|colspan="4" style = "font-size:90%; color:black;" |Typically expression of CD45 approaches that of normal T and B cells.
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''Major markers useful in diagnosis'''  
|-
|-
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker'''
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker'''
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|colspan="1" style = "font-size:80%; width: 80%; |'''Comment'''
|colspan="1" style = "font-size:80%; width: 80%; |'''Comment'''
|-
|-
|colspan="1" style = "width: 4%; font-size:90%; color:navy;" |<u>'''κ/λ'''</u>
|colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ
|colspan="1" style = "width: 8%; background:#006699;"|
|colspan="1" style = "width: 8%; background:#006699;"|
|colspan="1" style = "font-size:80%; width: 8%; |
|colspan="1" style = "font-size:80%; width: 8%; |
|colspan="1" style = "font-size:80%;"|Expect κ or λ expression, undetectable in rare cases
|colspan="1" style = "font-size:80%;"|Expect κ or λ expression, undetectable in rare cases
|-
|-
|colspan="1" style = "font-size:90%; color:navy;" |'''FMC7'''
|colspan="1" style = "font-size:90%; color:black;" |CD19
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|Occasionally seen, FMC7 is not typical of FL
|colspan="1" style = "font-size:84%;"|Expression is expected, but may be weak or undectable
|-
|-
|colspan="1" style = "font-size:90%; color:navy;" |<u>'''CD5'''</u>
|colspan="1" style = "font-size:90%; color:black;" |CD5
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|Expression of CD5 is characteristic of MCL
|colspan="1" style = "font-size:80%;"|Expression of CD5 is characteristic of MCL
|-
|-
|colspan="1" style = "font-size:90%; color:navy;" |<u>'''CD10'''</u>
|colspan="1" style = "font-size:90%; color:black;" |CD10
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|Strg
|colspan="1" style = "font-size:80%;"|Strg
|colspan="1" style = "font-size:84%;"|Expression is expected though not universal in FL
|colspan="1" style = "font-size:84%;"|Expression is expected though not universal in FL
|-
|colspan="1" style = "font-size:90%; color:black;" |CD23
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|May be expressed by some cases of FL
|-
|colspan="1" style = "font-size:90%; color:black;" |CD79b
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|
|colspan="1" style = "font-size:84%;"|Expression is expected and should be stronger than CLL
|-
|colspan="1" style = "font-size:90%; color:black;" |CD200
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Characteristically (though not always) negative in FL
|-
|colspan="1" style = "font-size:90%; color:black;" |FMC7
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|Occasionally seen, FMC7 is not typical of FL
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers
|-
|-
|colspan="1" style = "font-size:90%;" |CD11C
|colspan="1" style = "font-size:90%;" |CD11C
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|colspan="1" style = "font-size:80%;"|
|colspan="1" style = "font-size:80%;"|
|colspan="1" style = "font-size:84%;"|Expression is not typically seen in FL
|colspan="1" style = "font-size:84%;"|Expression is not typically seen in FL
|-
|colspan="1" style = "font-size:90%; color:navy;" |<u>'''CD19'''</u>
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression is expected, but may be weak or undectable
|-
|-
|colspan="1" style = "font-size:90%;" |CD20
|colspan="1" style = "font-size:90%;" |CD20
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|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression is expected, rare cases may have CD20 loss
|colspan="1" style = "font-size:84%;"|Expression is expected, rare cases may have CD20 loss
|-
|colspan="1" style = "font-size:90%; color:black;" |CD23
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|May be expressed by some cases of FL
|-
|-
|colspan="1" style = "font-size:90%; color:black;" |CD25
|colspan="1" style = "font-size:90%; color:black;" |CD25
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|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression is uncommon in FL, may be helpful in some cases
|colspan="1" style = "font-size:84%;"|Expression is uncommon in FL, may be helpful in some cases
|-
|colspan="1" style = "font-size:90%; color:black;" |CD79b
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|
|colspan="1" style = "font-size:84%;"|Expression is expected and should be stronger than CLL
|-
|-
|colspan="1" style = "font-size:90%;" |CD103
|colspan="1" style = "font-size:90%;" |CD103
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|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation  
|colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation  
|-
|colspan="1" style = "font-size:90%; color:navy;" |<u>'''CD200'''</u>
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Characteristically (though not always) negative in FL
|-
|-
|}
|}
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<span style="color:navy;font-size:80% ">'''Key to table: '''</span>
<span style="color:navy;font-size:80% ">'''Key to table: '''</span>
<span style="font-size:80% ">Markers most useful in initial diagnosis are <span style=color:navy><u>underlined in blue text</u></span></span>


<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span>
<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span>

Latest revision as of 17:17, 13 July 2023

  • FL.jpg


Background

Follicular lymphoma expresses CD10, with other immunophenotypic featues differing from related disorders.

However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing.


Immunophenotype of FL
CD45 expression
Typically expression of CD45 approaches that of normal T and B cells.
Major markers useful in diagnosis
Marker Freq Level Comment
κ/λ Expect κ or λ expression, undetectable in rare cases
CD19 - Expression is expected, but may be weak or undectable
CD5 - Expression of CD5 is characteristic of MCL
CD10 Strg Expression is expected though not universal in FL
CD23 - May be expressed by some cases of FL
CD79b Expression is expected and should be stronger than CLL
CD200 - Characteristically (though not always) negative in FL
FMC7 - Occasionally seen, FMC7 is not typical of FL
Other relevant markers
CD11C Expression is not typically seen in FL
CD20 - Expression is expected, rare cases may have CD20 loss
CD25 Not typical of FL, where present it is not generally strong
CD38 - Expression is expected in FL but CD138 should be absent
CD43 - Expression is uncommon in FL, may be helpful in some cases
CD103 - Not expressed by FL, consider HCL if found
CD138 - Predominantly expressed at plasma cell differentiation


Key to table:

Key to colour code for expression frequency Click for link

Key to expression strength code and use Click for link


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