Flow cytometry: Follicular Lymphoma: Difference between revisions
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!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of FL''' | !colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of FL''' | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | |||
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|colspan="4" style = "font-size:90%; color:black;" |Typically expression of CD45 is less intense than normal T or B cells, with many cases showing variable expression with a wide spread of CD45 intensity. | |||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''Major markers useful in diagnosis''' | !colspan="4" style = "font-size:90%; background:#DAE8F2"|'''Major markers useful in diagnosis''' | ||
Revision as of 17:15, 13 July 2023
Background
Follicular lymphoma expresses CD10, with other immunophenotypic featues differing from related disorders.
However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing.
| Immunophenotype of FL | |||
|---|---|---|---|
| CD45 expression | |||
| Typically expression of CD45 is less intense than normal T or B cells, with many cases showing variable expression with a wide spread of CD45 intensity. | |||
| Major markers useful in diagnosis | |||
| Marker | Freq | Level | Comment |
| κ/λ | Expect κ or λ expression, undetectable in rare cases | ||
| CD19 | - | Expression is expected, but may be weak or undectable | |
| CD5 | - | Expression of CD5 is characteristic of MCL | |
| CD10 | Strg | Expression is expected though not universal in FL | |
| CD23 | - | May be expressed by some cases of FL | |
| CD79b | Expression is expected and should be stronger than CLL | ||
| CD200 | - | Characteristically (though not always) negative in FL | |
| FMC7 | - | Occasionally seen, FMC7 is not typical of FL | |
| Other relevant markers | |||
| CD11C | Expression is not typically seen in FL | ||
| CD20 | - | Expression is expected, rare cases may have CD20 loss | |
| CD25 | Not typical of FL, where present it is not generally strong | ||
| CD38 | - | Expression is expected in FL but CD138 should be absent | |
| CD43 | - | Expression is uncommon in FL, may be helpful in some cases | |
| CD103 | - | Not expressed by FL, consider HCL if found | |
| CD138 | - | Predominantly expressed at plasma cell differentiation | |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link