Flow cytometry: Follicular Lymphoma: Difference between revisions
From haematologyetc.co.uk
No edit summary |
No edit summary |
||
Line 12: | Line 12: | ||
However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing. | However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing. | ||
'''CD45 Expression''' | |||
[[file:FCLCD45.jpg|300px|link=FCLCD45.jpg]] | |||
<span style="font-size:90%">''Side scatter of CLL cells is typically low, with a broad range and relatively weak expression of CD45 compared with normal lymphocytes. The CLL cells (highlighted in blue) therefore tend to the left of normal T cells (red) - giving the CLL population a rather elongated "slug-like" shape.'' | |||
Revision as of 21:52, 4 July 2023
Typical FCL morphology
Background
Follicular lymphoma expresses CD10, with other immunophenotypic featues differing from related disorders.
However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing.
CD45 Expression
Side scatter of CLL cells is typically low, with a broad range and relatively weak expression of CD45 compared with normal lymphocytes. The CLL cells (highlighted in blue) therefore tend to the left of normal T cells (red) - giving the CLL population a rather elongated "slug-like" shape.
Immunophenotype of FL | |||
---|---|---|---|
Major markers useful in CLL diagnosis | |||
Marker | Freq | Level | Comment |
κ/λ | Expect κ or λ expression, undetectable in rare cases | ||
FMC7 | - | Occasionally seen, FMC7 is not typical of FL | |
CD5 | - | Expression of CD5 is characteristic of MCL | |
CD10 | Strg | Expression is expected though not universal in FL | |
CD11C | Expression is not typically seen in FL | ||
CD19 | - | Expression is expected, but may be weak or undectable | |
CD20 | - | Expression is expected, rare cases may have CD20 loss | |
CD23 | - | May be expressed by some cases of FL | |
CD25 | Not typical of FL, where present it is not generally strong | ||
CD38 | - | Expression is expected in FL but CD138 should be absent | |
CD43 | - | Expression is uncommon in FL, may be helpful in some cases | |
CD79b | Expression is expected and should be stronger than CLL | ||
CD103 | - | Not expressed by FL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation | |
CD200 | - | Characteristically (though not always) negative in FL |
Key to table:
Markers most useful in initial diagnosis are underlined in blue text
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link