Flow cytometry: Diffuse Large B-cell Lymphoma: Difference between revisions
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(Created page with "---- 200px|link=BLBCL.jpg <span style="color: navy>'''Background to flow cytometric diagnosis'''</span> This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequet penotypic features, but emphasises that there is no absolutely typical pattern that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only...") |
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|colspan="1" style = "font-size:80%;"|Light chain | |colspan="1" style = "font-size:80%;"|Light chain restriction usually detected | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD5 | |colspan="1" style = "font-size:90%; color:black;" |CD5 | ||
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|colspan="1" style = "font-size:84%;"|Expect expression | |colspan="1" style = "font-size:84%;"|Expect expression, rarely it is absent | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD25 | |colspan="1" style = "font-size:90%; color:black;" |CD25 | ||
Revision as of 10:03, 13 July 2023
Background to flow cytometric diagnosis
This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequet penotypic features, but emphasises that there is no absolutely typical pattern that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease so in many cases a diagnosis has already been made. Where these cases present as a new diagnosis by blood or marrow diagnosis requires careful correllation with other features.
| Immunophenotype of DLBCL | |||
|---|---|---|---|
| Major markers useful in CLL diagnosis | |||
| Marker | Freq | Level | Comment |
| CD19 | - | Generally CD19 will be expressed | |
| κ/λ | Light chain restriction usually detected | ||
| CD5 | - | Uncommon but seen in some cases | |
| CD23 | - | Infrequent but reported | |
| CD79b | - | Generally expressed | |
| CD200 | - | Detected infrequently | |
| FMC7 | Generally expressed | ||
| Other relevant markers | |||
| CD10 | - | Expression may be seen | |
| CD11C | - | ||
| CD20 | - | Expect expression, rarely it is absent | |
| CD25 | - | ||
| CD38 | - | ||
| CD43 | - | ||
| CD103 | - | ||
| CD138 | - | ||
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link