Flow cytometric marker pattern of mantle cell lymphoma
From haematologyetc.co.uk
Background
Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200.
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm.
| Immunophenotype of MCL | |||
|---|---|---|---|
| Major markers useful in CLL diagnosis | |||
| Marker | Freq | Level | Comment |
| κ/λ | Expect moderate or strong restricted κ or λ expression | ||
| FMC7 | - | FMC7 is frequently expressed by typical MCL | |
| CD5 | - | Expression of CD5 is characteristic of MCL | |
| CD10 | - | Infrequently expressed, expression may suggest FL | |
| CD11C | Expressed in some cases of MCL but not typical | ||
| CD19 | - | Expression is expected, typically more strongly than in CLL | |
| CD20 | - | Expression is expected, typically stronger than CLL | |
| CD23 | - | Typically not expressed by MCL, may rarely be expressed | |
| CD25 | Not typical of MCL, not generally strong as HCL | ||
| CD38 | - | Expression is expected in MCL but CD138 should be absent | |
| CD43 | - | Lower expression than in CLL, may be helpful in some cases | |
| CD79a | Expression is expected and should be stronger than CLL | ||
| CD103 | - | Not expressed by MCL, consider HCL if found | |
| CD138 | - | Predominantly expressed at plasma cell differentiation | |
| CD200 | - | Characteristically (though not always) negative in MCL | |
Key to table:
Markers most useful in initial diagnosis are underlined in blue text
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link