Flow Cytometry:T-Lymphoid lineage assignment: Difference between revisions
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!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">|'''Assignment of T-lymphoid lineage'''</br></span> | |||
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|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" |For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br> | |||
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|colspan="1" style = "font-size:90%; color:black;"|'''Either''' [[CD3|CD3 surface or cytoplasm (strong)]] '''Or''' [[CD3|CD3 by immunocytochemistry (strong)]] | |||
|colspan="1" style = "font-size:90%;"|To meet the definition of "strong" the expression intensity '''must exceed''' that of 50% of normal T lymphocytes | |||
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<span style="font-size:85%;>'''Notes on interpretation of T-lineage in MPAL'''</br> | |||
Expression of CD3 is required to assign T lineage in MPAL. Generally, other T-associated antigens will also be expressed:.CD7, and in the majority of cases. CD2, CD4, CD5, and CD8. Consider also that a proportion of early T precursor lymphoblastic leukaemia/lymphoma (ETP-ALL) may be difficult to distinguish from MPAL-T/M. Using an appropriate threshold for MPO positivity may be helpful and a threshold of >10% for myeloperoxidase positivity is broadly advised by WHO in this context. | |||
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Latest revision as of 20:22, 5 January 2024
| Assignment of T-lymphoid lineage | |
|---|---|
| For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine. | |
| Either CD3 surface or cytoplasm (strong) Or CD3 by immunocytochemistry (strong) | To meet the definition of "strong" the expression intensity must exceed that of 50% of normal T lymphocytes |
Notes on interpretation of T-lineage in MPAL
Expression of CD3 is required to assign T lineage in MPAL. Generally, other T-associated antigens will also be expressed:.CD7, and in the majority of cases. CD2, CD4, CD5, and CD8. Consider also that a proportion of early T precursor lymphoblastic leukaemia/lymphoma (ETP-ALL) may be difficult to distinguish from MPAL-T/M. Using an appropriate threshold for MPO positivity may be helpful and a threshold of >10% for myeloperoxidase positivity is broadly advised by WHO in this context.