Flow Cytometry:T-Lymphoid lineage assignment: Difference between revisions
From haematologyetc.co.uk
No edit summary |
No edit summary |
||
| Line 2: | Line 2: | ||
<div style="width: 200px"> | <div style="width: 200px"> | ||
{| class="wikitable" style="border-left:solid 5px green;border-right:solid 5px green;border-top:solid 5px black;border-bottom:solid 5px black; font-size:90%; color:navy" | {| class="wikitable" style="border-left:solid 5px green;border-right:solid 5px green;border-top:solid 5px black;border-bottom:solid 5px black; font-size:90%; color:navy" | ||
| colspan="1"''|[[ | | colspan="1"''|[[Tables of diagnostic markers supporting lineage assignment in AML|Return to previous page]]'' | ||
|} | |} | ||
</div> | </div> | ||
Revision as of 12:58, 5 January 2024
| Requirements to assign T-lymphoid lineage
| |
|---|---|
| Marker requirement | |
| Either CD3 surface or cytoplasm (strong) Or CD3 by immunocytochemistry (strong) | To meet the definition of "strong" the expression intensity must exceed that of 50% of normal T lymphocytes |
Notes on interpretation of T-lineage in MPAL
Expression of CD3 is required to assign T lineage in MPAL. Generally, other T-associated antigens will also be expressed:.CD7, and in the majority of cases. CD2, CD4, CD5, and CD8. Consider also that a proportion of early T precursor lymphoblastic leukaemia/lymphoma (ETP-ALL) may be difficult to distinguish from MPAL-T/M. Using an appropriate threshold for MPO positivity may be helpful and a threshold of >10% for myeloperoxidase positivity is broadly advised by WHO in this context.