Flow Cytometry:T-Lymphoid lineage assignment: Difference between revisions
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!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">|'''Requirements to assign T-lymphoid lineage | !colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">|'''Requirements to assign T-lymphoid lineage'''</br></span> | ||
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Revision as of 14:35, 22 December 2023
| Requirements to assign T-lymphoid lineage
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|---|---|
| Marker requirement | |
| Either CD3 surface or cytoplasm (strong) Or CD3 by immunocytochemistry (strong) | To meet the definition of "strong" the expression intensity must exceed that of 50% of normal T lymphocytes |
Notes on interpretation of T-lineage in MPAL
Expression of CD3 is required to assign T lineage in MPAL. Generally, other T-associated antigens will also be expressed:.CD7, and in the majority of cases. CD2, CD4, CD5, and CD8. Consider also that a proportion of early T precursor lymphoblastic leukaemia/lymphoma (ETP-ALL) may be difficult to distinguish from MPAL-T/M. Using an appropriate threshold for MPO positivity may be helpful and a threshold of >10% for myeloperoxidase positivity is broadly advised by WHO in this context.