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mpo (myeloperoxidase) | mpo (myeloperoxidase) | ||
MPO expression is strongly associated with granulocytic maturation. In acute leukaemia MPO has high specificity for the diagnosis of AML, although expression may be observed on more primitive AML, or where maturation is non-granulocytic e.g. erythroid or megakaryocyte lineages and on some monocytic cases. Expression is not observed in typical ALL, if MPO is detected in ALL then this supports the diagnosis of mixed-phenotype acute leukaemia (MPAL). | |||
normal function and expression | normal function and expression | ||
Myeloperoxidase is a lysosomal enzyme used by neutrophils to kill pathogens. It is present in neutrophils and eosinophils at all stages of maturation, and in developing basophils. MPO may be expressed at low intensity in monocytes but erythroid precursors and lymphocytes are MPO-negative. | |||
what is its diagnostic role | what is its diagnostic role | ||
* | |||
*MPO is expressed in around 60% of AML cases - generally those with granulocytic maturation and is weakly expressed by promonocytes | |||
*In otherwise typical ALL a small number of MPO-expressing cells may be seen although precise criteria vary (most often <5%) | |||
*In cases of mixed phenotype acute leukaemia (MPAL), expression of mpo can alone be used to establish myeloid differentiation | |||
other relevant information | other relevant information | ||
Technically enzymatic measures of MPO (cytochemical or sudan black B staining) are probably the least sensitive detection methods. Flow cytometric detection of MPO has lower sensitivy than immunohistochemical detection on trephine; this may be because immunocytochemistry detects expression of a precursor enzyme form of MPO as well as thefunctional form. For this reason, if there is doubt about MPO expression then MPO staining of trephine biopsies can be useful. | |||
summary tables | summary tables | ||
Revision as of 09:42, 31 May 2023
mpo (myeloperoxidase)
MPO expression is strongly associated with granulocytic maturation. In acute leukaemia MPO has high specificity for the diagnosis of AML, although expression may be observed on more primitive AML, or where maturation is non-granulocytic e.g. erythroid or megakaryocyte lineages and on some monocytic cases. Expression is not observed in typical ALL, if MPO is detected in ALL then this supports the diagnosis of mixed-phenotype acute leukaemia (MPAL).
normal function and expression
Myeloperoxidase is a lysosomal enzyme used by neutrophils to kill pathogens. It is present in neutrophils and eosinophils at all stages of maturation, and in developing basophils. MPO may be expressed at low intensity in monocytes but erythroid precursors and lymphocytes are MPO-negative.
what is its diagnostic role
- MPO is expressed in around 60% of AML cases - generally those with granulocytic maturation and is weakly expressed by promonocytes
- In otherwise typical ALL a small number of MPO-expressing cells may be seen although precise criteria vary (most often <5%)
- In cases of mixed phenotype acute leukaemia (MPAL), expression of mpo can alone be used to establish myeloid differentiation
other relevant information Technically enzymatic measures of MPO (cytochemical or sudan black B staining) are probably the least sensitive detection methods. Flow cytometric detection of MPO has lower sensitivy than immunohistochemical detection on trephine; this may be because immunocytochemistry detects expression of a precursor enzyme form of MPO as well as thefunctional form. For this reason, if there is doubt about MPO expression then MPO staining of trephine biopsies can be useful.
summary tables
expression by acute leukaemias and by haematogones rare pro-ball rare early pre-ball rare pre-ball rare ball rare pro-tall rare mature-tall mod aml * rare haemato-gones
- *inamlmpoishighly specific fordiagnosis butisnotdetected inallcases 2.
expression byb-lymphoproliferative disorders rare cll* rare pll rare mcl rare fl rare hcl rare hclv rare mzl rare lpl rare pcs