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Revision as of 09:17, 15 May 2023

Summary

Principally a marker of cell acTivaTion in differenT haemaTopoieTic lineages CD25 has value in several disorders Though in each case be aware ThaT iT is noT absoluTely specific

CD25 has recognised value in The diagnosis of hairy cell leukaemia
CD25 recognises malignanT masT cells
iT may be valuable To idenTify The malignanT cells of adulT T cell leukaemia lymphoma

Normal expression and function

CD25 is The alpha subuniT of The inTerleukin 2 recepTor (IL2). When associaTed wiTh iTs beta subunit, memory T cells or NK cells or wiTh beta and gamma subunits expressed by activaTed T cells or regulatory T cells, the recepTor affiniTy for il-2 is progressively increased and CD25 activates intracellular signals causing cellular activation. CD25 has a broad expression in the immune system u8e28093 mosT frequently following cell activation.

Diagnostic role

CD25 may be expressed by a range of malignant cells including for example many cases of CLL . It does however have value in specific circumsTances

CD25 is expressed by most cases of hairy cell leukemia and is not generally expressed by variant hairy cell leukemia or splenic marginal zone lymphoma
The malignanT mast cells of sysTemic mastocyTosis express CD25 normal mast cells generally do not
CD25 is strongly expressed by The neoplastic T cells in adulT T cell leukemia lymphoma ATLL alThough it is expressed also in oTher T cell disorders notably T-PLL and sezary syndrome
CD25 should noT be considered as lineage or sTage-specific. For example expression is seen both in AML possibly 10% and in ALL 30% - parTicularly in cases expressing BCR::ABL1

Other relevant information:

In a histlogical conText The marker has a wider diagnostic use in tissue lymphoma sections, staining is seen in anaplasTic large cell lymphoma wiTh high frequency and in Hodgkin disease.

SUMMARY TABLES

Note The colour and % refer to the proportion of cases expressing the marker. Additional comments on strength, or site of expression (if relevant) are given below the table. For full details of key click here. For further information about a particular disease state click <I> adjacent to the condition name

Expression in primitive cell types
AML	CMML	B ALL	Burkitt	T ALL	ETP ALL <I>	MPAL <I>	Hgns <I>
? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%

Notes: xxxxx

Expression in mature B cell neoplasms
CLL	MCL	FL	MZL	HCL	DLBCL	LPL	PCL
? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%

Notes: xxxxx

Expression in mature T cell neoplasms*
ATLL	CTCL/Sezary	T-PLL	T-LGL	NK-LGL
? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%	? <5% 5-20% 20-40% 40-80% 80-100%

Notes: xxxxx

@@ Line 5: / Line 5: @@
 :<span style="color:navy">'''Summary'''</span>
-:In the detection of haematological malignancy the purpose of detecting γδ T cells is to define particular T cell lymphomas (αβ T cells strongly predominate in normal blood). However care must be taken when small populations of γδ T cells are detected however, since these may arise in response to viral or other immune responses.
+:Principally a marker of cell acTivaTion in differenT haemaTopoieTic lineages CD25 has value in several disorders Though in each case be aware ThaT iT is noT absoluTely specific
+*CD25 has recognised value in The diagnosis of hairy cell leukaemia
+*CD25 recognises malignanT masT cells
+*iT may be valuable To idenTify The malignanT cells of adulT T cell leukaemia lymphoma
+</div>
+<span style="color:navy">'''Normal expression and function'''</span>
+CD25 is The alpha subuniT of The inTerleukin 2 recepTor (IL2). When associaTed wiTh iTs beta subunit, memory T cells or NK cells or wiTh beta and gamma subunits expressed by activaTed T cells or regulatory T cells, the recepTor affiniTy for il-2 is progressively increased and CD25 activates intracellular signals causing cellular activation.
+CD25 has a broad expression in the immune system u8e28093 mosT frequently following cell activation.
+<span style="color:navy">'''Diagnostic role'''</span>
+CD25 may be expressed by a range of malignant cells including for example many cases of CLL . It does however have value in specific circumsTances
+* CD25 is expressed by most cases of hairy cell leukemia and is not generally expressed by variant hairy cell leukemia or splenic marginal zone lymphoma
+* The malignanT mast cells of sysTemic mastocyTosis express CD25 normal mast cells generally do not
+* CD25 is strongly expressed by The neoplastic T cells in adulT T cell leukemia lymphoma ATLL alThough it is expressed also in oTher T cell disorders notably T-PLL and sezary syndrome
+* CD25 should noT be considered as lineage or sTage-specific. For example expression is seen both in AML possibly 10% and in ALL 30% - parTicularly in cases expressing  BCR::ABL1
-</div>
+<span style="color:navy">'''Other relevant information:'''</span>
+In a histlogical conText The marker has a wider diagnostic use in tissue lymphoma sections, staining is seen in anaplasTic large cell lymphoma wiTh high frequency and in Hodgkin disease.
+----
+<span style="color:navy">'''''SUMMARY TABLES'''''</span>
+<span style="font-size:90%">'''Note''' The colour and % refer to the proportion of cases expressing the marker. Additional comments on strength, or site of expression (if relevant) are given below the table. For full details of key click [[Key to marker expression table|here]]. For further information about a particular disease state click <<span style="color:blue>'''I'''</span>> adjacent to the condition name</span>
+{| class=wikitable style="text-align: center; font-size:80%; width: 100%; height 20px;"
+|-
+!colspan="8"|<span style="font-size:100%">'''Expression in primitive cell types'''</font>
+|-
+! AML !! CMML !! B ALL !! Burkitt || T ALL !! ETP ALL <'''[[ETP ALL|I]]'''> !! MPAL <'''[[MPAL|I]]'''> || Hgns <'''[[Hgns|I]]'''>
+|-
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|-
+|}
+<span style="font-size:90%">'''Notes:''' xxxxx </span>
-:<span style="color:navy">'''Background'''</span>
+{| class=wikitable style="text-align: center; font-size:80%; width: 100%; height 20px;"
+|-
+!colspan="8"|'''Expression in mature B cell neoplasms'''
+|-
+! CLL !! MCL !!FL!! MZL !! HCL !! DLBCL || LPL !! PCL
+|-
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 12.5%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|-
+|}
-:T cell receptor (TCR) analysis in blood is not generally helpful in indicating T-cell clonality since around 95% express of circulating T cells express αβ chains and only 5% have gamma γδ. In tissues this can be different since the γδ T cells are more abundant in skin and intraepithelial areas. The receptors also have different functions:  αβ T cells behave as elements of the adaptive immune system while γδ cells have an innate immune function similar to NK cells.
+<span style="font-size:90%">'''Notes:''' xxxxx </span>
-:<span style="color:navy">'''Diagnostic Use'''</span>
-*A predominant population of γδ T cells circulate may indicate a clonal proliferation most commonly will be hepatosplenic gamma-delta T-cell lymphoma  or primary cutaneous t cell lymphoma (PTCL)
+{| class=wikitable style="text-align: center; font-size:80%; width: 62.4%; height 40px;"
-*Disturbance of the ratio of αβ/γδ may be seen in reactive conditions
+|-
+!colspan="7"|'''Expression in mature T cell neoplasms*'''
+|-
+! ATLL !! CTCL/Sezary !! T-PLL !! T-LGL !! NK-LGL
+|-
+|style="width: 14.25%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 14.25%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 14.25%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 14.25%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|style="width: 14.25%; background: ?#FFE4E1 5#E6FAFF 20#66e0ff 40#00b8e6 80#006699;100#004466; color:white black"|? <5% 5-20% 20-40% 40-80% 80-100%
+|-
+|}
+<span style="font-size:90%">'''Notes:''' xxxxx </span>
------
+----

Extra: Difference between revisions

From haematologyetc.co.uk

Revision as of 09:17, 15 May 2023