Actions

CD14: Difference between revisions

From haematologyetc.co.uk

(Created page with "(259, 'cd14', ' cd14 aids recognition ofu800 bacteria byu800 cells ofu800 monocytic lineage. itu800 mayu800 generally beu800 regarded asu800 au800 panu800-monocyte antigen * when expressed inu800 amlu800 cd14 isu800 au800 marker ofu800 monocytic lineage * absence ofu800 cd14 expression does notu800 preclude monocytic lineage since some typical monoblasts dou800 notu800 express this marker * cd14 isu800 notu800 restricted tou800 malignant cells andu800 will beu800 express...")
 
No edit summary
 
(25 intermediate revisions by the same user not shown)
Line 1: Line 1:
(259, 'cd14', ' cd14 aids recognition ofu800 bacteria byu800 cells ofu800 monocytic lineage. itu800 mayu800 generally beu800 regarded asu800 au800 panu800-monocyte antigen * when expressed inu800 amlu800 cd14 isu800 au800 marker ofu800 monocytic lineage * absence ofu800 cd14 expression does notu800 preclude monocytic lineage since some typical monoblasts dou800 notu800 express this marker * cd14 isu800 notu800 restricted tou800 malignant cells andu800 will beu800 expressed byu800 reactive monocytes andu800 also byu800 neoplastic cells ofu800 cmml normal expression andu800 function together with cou800-receptors cell surface cd14 binds theu800 bacterial cell wall component lipopolysaccharide lpsu800 helping initiate cytokine secretion andu800 theu800 inflammatory response when bacteria areu800 detected. cd14 isu800 anchored tou800 theu800 membrane byu800 au800 glycosylphosphatidylinositol gpiu800 tail attachment mayu800 beu800 cleaved tou800 release au800 soluble form. cd14 isu800 expressed mainly byu800 monocytes dendritic cells andu800 macrophages andu800 tou800 au800 much lower extent byu800 neutrophils . cd14 isu800 weakly expressed oru800 absent onu800 early monocytes butu800 expression becomes progressively stronger asu800 theu800 cells mature.  diagnostic role cd14 isu800 au800 useful marker ofu800 monocytic lineage inu800 amlu800. *cases with myelomonocytic lineage will express cd14 onu800 theu800 monocytic population ofu800 blasts *cases with pure monocytic differentiation will generally express cd14 although itu800 mayu800 beu800 absent onu800 less mature monoblasts. *expression isu800 usual onu800 cmml monocytes sou800 itu800 does notu800 distinguish cmml andu800 monocytic amlu800. *expression isu800 very uncommon onu800 lymphoid lineage with 5u800 expression byu800 allu800 cells. other relevant information   cd14 isu800 glycophosphoinositol gpiu800 linked andu800 will therefore beu800 absent inu800 cells affected byu800 paraxysmol nocturnal haemoglobinuria pnhu800 . ---- summary tables  expression byu800 acute leukaemias andu800 byu800 haematogones file rareu82epngu800  prou800-bu800 allu800  link flow cytometry keyu800 file rareu82epngu800  early preu800-bu800 allu800  link flow cytometry keyu800 file rareu82epngu800  preu800-bu800 allu800  link flow cytometry keyu800 file rareu82epngu800  bu800 allu800  link flow cytometry keyu800 file rareu82epngu800  prou800-tu800 allu800  link flow cytometry keyu800 file rareu82epngu800  mature-tu800 allu800  link flow cytometry keyu800 file frequ82epngu800  amlu800  link flow cytometry keyu800 file rareu82epngu800  haemato-gones  link flow cytometry keyu800  notes  **inu800 amlu800 cd14 expression defines monocytic lineage restriction butu800 mayu800 beu800 absent especially inu800 more primitive monoblastic cases expression byu800 bu800-lymphoproliferative disorders file rareu82epngu800 frame  cllu800  link flow cytometry keyu800 file rareu82epngu800  pllu800  link flow cytometry keyu800 file rareu82epngu800  mclu800  link flow cytometry keyu800 file rareu82epngu800  flu800\' link flow cytometry keyu800 file rareu82epngu800  hclu800  link flow cytometry keyu800 file rareu82epngu800  hclv  link flow cytometry keyu800 file rareu82epngu800  mzlu800  link flow cytometry keyu800 file rareu82epngu800  lplu800  link flow cytometry keyu800 file rareu82epngu800  pcsu800  link flow cytometry keyu800 ---- keyu800  theu800 indicated expression frequency indicates theu800 approximate frequency ofu800 occurrence inu800 each particular disease type. file rareu82epngu800 30px link rare 5u800 file possu82epngu800 30px link possible 5u800-20u800 file modu800u82epngu800 30px link likely 20u800-40u800 file frequ82epngu800 30px link frequent 40u800-80u800 file hiu800u82epngu800 30px link expected 80u800 where expression level isu800 unusual oru800 characteristic ofu800 au800 disorder this isu800 indicated byu800 text ---- '),
 
<div style="background-color: #E0EEEE; {{round corners}} padding: 2em 2em;  margin-right: 5px;">
 
 
:<span style="color:navy">'''Summary'''</span>
 
:CD14 aids recognition of bacteria by cells of monocytic lineage, it is regarded as a pan-monocyte antigen. Expression of CD14 is not restricted to malignant cells as it is also expressed by normal and reactive monocytes 
 
:when expressed in aml CD14 is a marker of monocytic lineage, but absence of CD14 expression does not preclude monocytic lineage in AML and CMML since some typical monoblasts may not express this marker  
 
 
   
</div>
 
 
 
<span style="color:navy">'''Normal expression and function'''</span>
 
Together with co-receptors cell surface, CD14 binds the bacterial cell wall component lipopolysaccharide (LPS) helping initiate cytokine secretion and the inflammatory response when bacteria are detected. CD14 is expressed mainly by monocytes dendritic cells and macrophages and to a much lower extent by neutrophils. CD14 is weakly expressed or absent on early monocytes, but expression becomes progressively stronger as the cells mature.   
 
 
 
<span style="color:navy">'''Diagnostic role'''</span>
 
*CD14 is a useful marker of monocytic lineage in aml
 
*CD14 may be absent on less mature monoblasts, while in myelomonocytic lineage cells will express CD14 on the monocytic population of blasts  
 
*In cmml expression of CD14 is expected, so it does not distinguish cmml and monocytic aml
 
*Expression is very uncommon on lymphoid lineage   
 
 
 
 
<span style="color:navy">'''Other relevant information:'''</span>
 
CD14 is glycophosphoinositol gpi linked and will therefore be absent in cells affected by paraxysmol nocturnal haemoglobinuria (PNH)
 
 
----
 
<span style="color:navy">'''''SUMMARY TABLES'''''</span>
 
 
<span style="font-size:90%">'''Note''' The colour and % refer to the proportion of cases expressing the marker. Additional comments on strength, or site of expression (if relevant) are given below the table. For full details of key click [[Key to marker expression table|here]]. For further information about a particular disease state click <<span style="color:blue>'''I'''</span>> adjacent to the condition name</span>
 
 
{| class=wikitable style="text-align: center; font-size:80%; width: 100%; height 20px;"
|-
!colspan="8"|'''Expression in primitive cell types'''</font>
|- <span style="font-size:70%">
!AML!!CMML !!B ALL !!BL ||T ALL !!ETP ALL !!MPAL ||Hgn</span>
|-<span style="font-size:70%">
|style="width: 12.5%; background: #66e0ff; color:black"|20-40%
|style="width: 12.5%; background: #004466; color:white"|80-100%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|-
|}
 
<span style="font-size:90%">'''Notes:''' In aml CD14 expression defines monocytic lineage restriction but may be absent especially in more primitive monoblastic cases   </span>
 
 
{| class=wikitable style="text-align: center; font-size:80%; width: 100%; height 20px;"
|-
!colspan="8"|'''Expression in mature B cell neoplasms'''
|- <span style="font-size:70%">
! CLL !! MCL !!FL!! MZL !! HCL !! DLBCL || LPL !! PCL
|-<span style="font-size:70%">
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|style="width: 12.5%; background: #E6FAFF; color:black"|<5%
|-
|}
 
<span style="font-size:90%">'''Notes:''' Low level expression has been reported on activated lymphocytes, but it has no definite diagnostic role </span>
 
 
 
{| class=wikitable style="text-align: center; font-size:80%; width: 62.4%; height 40px;"
|-
!colspan="7"|'''Expression in mature T cell neoplasms'''
|-  
! ATLL !! CTCL/Sezary !! T-PLL !! T-LGL !! NK-LGL
|-
|style="width: 14.25%; background: #E6FAFF; color:black"|<5%
|style="width: 14.25%; background: #E6FAFF; color:black"|<5%
|style="width: 14.25%; background: #E6FAFF; color:black"|<5%
|style="width: 14.25%; background: #E6FAFF; color:black"|<5%
|style="width: 14.25%; background: #E6FAFF; color:black"|<5%
|-
|}
 
 
----

Latest revision as of 21:38, 4 July 2023


Summary
CD14 aids recognition of bacteria by cells of monocytic lineage, it is regarded as a pan-monocyte antigen. Expression of CD14 is not restricted to malignant cells as it is also expressed by normal and reactive monocytes
when expressed in aml CD14 is a marker of monocytic lineage, but absence of CD14 expression does not preclude monocytic lineage in AML and CMML since some typical monoblasts may not express this marker



Normal expression and function

Together with co-receptors cell surface, CD14 binds the bacterial cell wall component lipopolysaccharide (LPS) helping initiate cytokine secretion and the inflammatory response when bacteria are detected. CD14 is expressed mainly by monocytes dendritic cells and macrophages and to a much lower extent by neutrophils. CD14 is weakly expressed or absent on early monocytes, but expression becomes progressively stronger as the cells mature.


Diagnostic role

  • CD14 is a useful marker of monocytic lineage in aml
  • CD14 may be absent on less mature monoblasts, while in myelomonocytic lineage cells will express CD14 on the monocytic population of blasts
  • In cmml expression of CD14 is expected, so it does not distinguish cmml and monocytic aml
  • Expression is very uncommon on lymphoid lineage



Other relevant information:

CD14 is glycophosphoinositol gpi linked and will therefore be absent in cells affected by paraxysmol nocturnal haemoglobinuria (PNH)


SUMMARY TABLES


Note The colour and % refer to the proportion of cases expressing the marker. Additional comments on strength, or site of expression (if relevant) are given below the table. For full details of key click here. For further information about a particular disease state click <I> adjacent to the condition name


Expression in primitive cell types
AML CMML B ALL BL T ALL ETP ALL MPAL Hgn
20-40% 80-100% <5% <5% <5% <5% <5% <5%

Notes: In aml CD14 expression defines monocytic lineage restriction but may be absent especially in more primitive monoblastic cases


Expression in mature B cell neoplasms
CLL MCL FL MZL HCL DLBCL LPL PCL
<5% <5% <5% <5% <5% <5% <5% <5%

Notes: Low level expression has been reported on activated lymphocytes, but it has no definite diagnostic role


Expression in mature T cell neoplasms
ATLL CTCL/Sezary T-PLL T-LGL NK-LGL
<5% <5% <5% <5% <5%


Retrieved from ‘https://haematologyetc.co.uk/index.php?title=CD14&oldid=2227’