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| | <div style="background-color: #E0EEEE; {{round corners}} padding: 2em 2em; margin-right: 5px;"> |
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| | :CD3 is a reliable marker of T cell lineage that is primarily used to distinguish between T and B cells. The pattern of expression has diagnostic relevance. |
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| <span style="color:navy>1. ''Markers used mainly for gating:''</span> | | </div> |
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| | <span style="color:navy">'''Normal expression and function'''</span> |
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| | CD3 is a multi-molecular complex that associates with the T cell receptor of mature T cells, and supports TCR expression and intracellular signalling in response to antigen. It is first expressed in the cytoplasm of pro-thymocytes before migrating to the T-cell surface during thymic maturation. CD3 expression is almost exclusive to mature T cells (there is weak expression of CD3 by some macrophages). |
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| | <span style="color:navy">'''Diagnostic role'''</span> |
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| | * '''T-ALL''''' Expression is mainly cytoplasmic (cyCD3). Surface (sCD3) is usually absent in Early T-precursor T-ALL but is variably expressed in more mature T-ALL. |
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| | * '''Mature T cell neoplasms''': sCD3 is expressed on T cell lymphoproliferative disorders. The level of CD3 expression is increased (compared to normal T cells) in T-PLL and T-LGL, and is decreased in Sézary syndrome and ATLL |
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| | * '''Natural Killer cell lymphoproliferative disorders''': do not express a conventional TCR so CD3 is not expressed. |
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| | * '''Other''' B cells rarely express CD3, and aberrant expression in AML is rare (in contrast to other T cell markers such as CD2 or CD7). |
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| {|style="width: 80px; height: 80px; text-align:center; font-style: bold; font-size: 120%; color: teal; background: #ededed; border-style: solid; border-width: 2px"
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| [[CD45|<span style="color:teal">'''CD45'''</span>]]
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| |}
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| ---- | | ---- |
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| | <span style="color:navy">'''''SUMMARY TABLES'''''</span> |
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| | Expression by acute leukaemias and by haematogones |
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| | <gallery widths="40px" heights="40px" > |
| | File:rare.png|''Pro-B ALL''|link=Flow cytometry key |
| | File:rare.png|''Early pre-B ALL''|link=Flow cytometry key |
| | File:rare.png|''Pre-B ALL''|link=Flow cytometry key |
| | File:rare.png|''B ALL''|link=Flow cytometry key |
| | File:Hi.png|''Pro-T ALL''|link=Flow cytometry key |
| | File:Hi.png|''Mature-T ALL''|link=Flow cytometry key |
| | File:Poss.png|''AML'' *|link=Flow cytometry key |
| | File:Rare.png|''Haemato-gones''|link=Flow cytometry key |
| | </gallery> |
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| | <span style="font-size:80%">'''*''' May be seen in AML but consider bi-phenotypic if present</span> |
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| | Expression by B-lymphoproliferative disorders |
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| | <gallery widths="40px" heights="40px" > |
| | File:rare.png|frame|''CLL''|link=Flow cytometry key |
| | File:rare.png|''PLL''|link=Flow cytometry key |
| | File:rare.png|''MCL''|link=Flow cytometry key |
| | File:Poss.png|''FL'' *|link=Flow cytometry key |
| | File:rare.png|''HCL''|link=Flow cytometry key |
| | File:rare.png|''HCLv''|link=Flow cytometry key |
| | File:rare.png|''MZL''|link=Flow cytometry key |
| | File:rare.png|''LPL''|link=Flow cytometry key |
| | File:rare.png|''PCs''|link=Flow cytometry key |
| | </gallery> |
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| | <span style="font-size:80%">'''*''' occasionally described in FL</span> |
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| | Expression by mature T-cell disorders |
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| <span style="color:navy>2. ''Non CD-classified markers''</span> | | <gallery widths="40px" heights="40px" > |
| | File:Hi.png|frame|''T-PLL''|link=Flow cytometry key |
| | File:Hi.png|''T-LGL''|link=Flow cytometry key |
| | File:Rare.png|''NK-LGL''|link=Flow cytometry key |
| | File:Hi.png|''ATLL'' *|link=Flow cytometry key |
| | File:Hi.png|''Sezary'' *|link=Flow cytometry key |
| | File:Freq.png|''Others'' **|link=Flow cytometry key |
| | </gallery> |
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| | <span style="font-size:80%">'''*''' expression may be dim</span> |
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| {|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: maroon; background: #faeed8; border-style: solid; border-width: 2px; margin: 5px"
| | <span style="font-size:80%">'''**''' a range of other T cell disorders may circulate occasionally, marker expression varies</span> |
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| '''[[Immunoglobulin light chains|<span style="color:maroon">'''kappa'''</span>]]'''
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| |}
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| {|style="float:left; width: 80px; height: 80px; text-align:center; font-style: bold; color: maroon; background: #faeed8; border-style: solid; border-width: 2px; margin: 5px"
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| '''[[Immunoglobulin light chains|<span style="color:maroon">'''lambda'''</span>]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: navy; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[FMC7]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: navy; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[TDT]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: italic; color: navy; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[MPO]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: navy; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[IgM]]'''
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| |}
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| ---- | | ---- |
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| | '''KEY''' |
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| | <div class="toccolours mw-collapsible mw-collapsed" style="font-size 80%; overflow:auto;"> |
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| 3. '''CD-classified markers frequently used in leukaemia diagnosis'''
| | The indicated expression frequency, indicates the approximate frequency of occurrence in each particular disease type. |
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD2]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD3]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD5]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD10]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD11c]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD13]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD14]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color:teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD19]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD22]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD23]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD25]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD33]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD34]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal;; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD38]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD79a]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD79b]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD103]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD117]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD138]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD200]]'''
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| |}
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| | [[File:Rare.png|30px|link=]] |
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| ---- | | rare (<5%) |
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| | [[File:Poss.png|30px|link=]] |
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| | possible (5-20%) |
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| | [[File:Mod.png|30px|link=]] |
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| | likely (20-40%) |
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| | [[File:Freq.png|30px|link=]] |
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| | frequent (40-80%) |
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| | [[File:Hi.png|30px|link=]] |
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| | expected (>80%) |
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| 4. '''Other markers helpful in diagnosis'''
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: navy; border-style: solid; border-width: 15px; margin: 5px"
| | Where expression level is unusual or characteristic of a disorder this is indicated by text: |
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| '''[[TCRαβ]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: navy; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[TCRγδ]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: navy; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[HLA-DR]]'''
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| |}
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| <BR clear=all>
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
| | </div> |
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| '''[[CD1a]]'''*
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD4]]'''*
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD7]]'''*
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD8]]'''*
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD11b]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD15]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD16]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD20]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD24]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD41]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD43]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD56]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD57]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD61]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD64]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD65]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD123]]'''
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| |}
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| {|style="float:left; width: 100px; height: 100px; text-align:center; font-style: bold; color: teal; border-style: solid; border-width: 15px; margin: 5px"
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| '''[[CD235]]'''
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| |}
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| ---- | | ---- |
