Flow cytomtery: Lymphoplasmacytic lymphoma
From haematologyetc.co.uk
Background to flow cytometric diagnosis
Cells of LPL share features of mature B-cell differentation and features of early plasma cell differentiation, and have mixed populations with features of each (although proportions vary). This can be a challenging diagnosis by flow cytometry alone, but other modalities can generally make this diagnosis clear.
| Immunophenotype of LPL | |||
|---|---|---|---|
| CD45 expression | |||
| Typically expression of CD45 is similar to that of normal T and B cells. | |||
| Major markers useful in LPL diagnosis | |||
| Marker | Freq | Level | Comment |
| CD19 | - | Generally CD19 will be expressed | |
| CD38 | - | ||
| κ/λ | Light chain retriction usually detected | ||
| CD5 | - | Uncommon but seen in some cases | |
| CD138 | var | Particularly on cells with plasma cell features | |
| CD200 | - | Detected infrequently | |
| FMC7 | Generally expressed | ||
| Other relevant markers | |||
| CD10 | - | Expression may be seen | |
| CD11C | - | ||
| CD20 | - | Expect expression | |
| CD23 | - | Infrequent but reported | |
| CD25 | - | ||
| CD43 | - | Generally not expressed by LPL | |
| CD79b | - | Generally expressed | |
| CD103 | - | ||
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link