Flow cytometry: Hairy Cell Leukaemia - Revision history

John at 15:19, 13 July 2023

2023-07-13T15:19:24Z

← Older revision		Revision as of 17:19, 13 July 2023
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	<div style="width: 95%; overflow: auto; border: 1px solid navy; font-size:100%">		<div style="width: 95%; overflow: auto; border: 1px solid navy; font-size:100%">
	{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"		{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"
	!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype'''		!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of HCL'''
			\|-
			!colspan="4" style = "font-size:90%; background:#DAE8F2"\|'''CD45''' expression
			\|-
			\|colspan="4" style = "font-size:90%; color:black;" \|Typically expression of CD45 is high in HCL and is often gretaer than normal T and B cells.
	\|-		\|-
	!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in diagnosis		!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in diagnosis

John at 12:09, 13 July 2023

2023-07-13T12:09:00Z

← Older revision		Revision as of 14:09, 13 July 2023
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	----		----
			<gallery mode="nolines" widths="200px" heights="200px" border="1px" >
	[[file:HCL.jpg~~\|200px~~\|link=HCL.jpg]]		file:HCL.jpg\|link={{filepath:HCL.jpg}}
			</gallery>
			----

John at 19:14, 12 July 2023

2023-07-12T19:14:44Z

← Older revision		Revision as of 21:14, 12 July 2023
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	[[file:HCL.jpg\|200px\|link=HCL.jpg]]		[[file:HCL.jpg\|200px\|link=HCL.jpg]]

	<span style="font-size:90%">''Typical HCL morphology, cells are medium size, with a partly activated chromatin pattern and oval or indented nuclear shape, the cytoplasm is typically blue/grey with irregular and fine projections (hairs)''</span>

John at 19:14, 12 July 2023

2023-07-12T19:14:30Z

← Older revision		Revision as of 21:14, 12 July 2023
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	However, no marker is unique to HCL and unusual marker patterns can lead to diagnostic difficulty. If this is the case then the atypical features should be reported, since histopathology and molecular testing can each be used to further evaluate these cases.		However, no marker is unique to HCL and unusual marker patterns can lead to diagnostic difficulty. If this is the case then the atypical features should be reported, since histopathology and molecular testing can each be used to further evaluate these cases.


	~~'''CD45 expression'''~~


	~~[[file:HCLLCD45.jpg\|300px\|link=HCLCD45.jpg]]~~


	~~<span style="font-size:90%">blah blah</span>~~

John at 20:24, 4 July 2023

2023-07-04T20:24:12Z

John at 20:22, 4 July 2023

2023-07-04T20:22:38Z

← Older revision		Revision as of 22:22, 4 July 2023
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	<span style="color:navy;font-size:80% ">'''Key to table: '''</span>		<span style="color:navy;font-size:80% ">'''Key to table: '''</span>

	~~<span style="font-size:80% ">Markers most useful in initial diagnosis are <span style=color:navy><u>underlined in blue text</u></span></span>~~

	<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table\|Click for link]]</span>		<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table\|Click for link]]</span>

John: Created page with "---- link=HCL.jpg ''Typical HCL morphology, cells are medium size, with a partly activated chromatin pattern and oval or indented nuclear shape, the cytoplasm is typically blue/grey with irregular and fine projections (hairs)''
2023-06-30T09:10:37Z

Created page with "---- 200px|link=HCL.jpg ''Typical HCL morphology, cells are medium size, with a partly activated chromatin pattern and oval or indented nuclear shape, the cytoplasm is typically blue/grey with irregular and fine projections (hairs)'' '''Background to flow cytometric diagnosis''' HCL expresses typical and unusual markers that together with morphology can allow diagnosed with some co..."

New page
----

[[file:HCL.jpg|200px|link=HCL.jpg]]

''Typical HCL morphology, cells are medium size, with a partly activated chromatin pattern and oval or indented nuclear shape, the cytoplasm is typically blue/grey with irregular and fine projections (hairs)''

'''Background to flow cytometric diagnosis'''

HCL expresses typical and unusual markers that together with morphology can allow diagnosed with some confidence using flow cytometry.

However, no marker is unique to HCL and unusual marker patterns can lead to diagnostic difficulty. If this is the case then the atypical features should be reported, since histopathology and molecular testing can each be used to further evaluate these cases.

'''CD45 expression'''

[[file:CLLCD45.jpg|300px|link=CLLCD45.jpg]]

blah blah

<div style="width: 95%; overflow: auto; border: 1px solid navy; font-size:100%">
{| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"
!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype'''
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in diagnosis
|-
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker'''
|colspan="1" style = "font-size:80%; width: 8%; |'''Freq'''
|colspan="1" style = "font-size:80%; width: 8%; |'''Level'''
|colspan="1" style = "font-size:80%; width: 80%; |'''Comment'''
|-
|colspan="1" style = "width: 4%; font-size:90%; color:black;" |'''κ/λ'''
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%; width: 8%; |Stg
|colspan="1" style = "font-size:80%;"|Expect strong restricted expression of κ or λ
|-
|colspan="1" style = "font-size:90%; color:black;" |'''CD19'''
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression is expected and may be strong in HCL
|-
|colspan="1" style = "font-size:90%;" |'''CD11C'''
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|Stg
|colspan="1" style = "font-size:84%;"|Strong expression of CD11c is typical of HCL
|-
|colspan="1" style = "font-size:90%; color:black;" |'''CD25'''
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|wk
|colspan="1" style = "font-size:84%;"|A typical and most frequently strongly expressed marker
|-
|colspan="1" style = "font-size:90%; color:black;" |'''FMC7'''
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|Stg
|colspan="1" style = "font-size:80%;"|Typically this marker is strongly expressed
|-
|colspan="1" style = "font-size:90%;" |'''CD103'''
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|A characteristic marker of HCL with good specificity
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other useful markers
|-
|colspan="1" style = "font-size:90%; color:black;" |CD5
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|Infrequent but reported in some cases
|-
|colspan="1" style = "font-size:90%;" |CD10
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Infrequently expressed in typical HCL
|-
|colspan="1" style = "font-size:90%;" |CD20
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|Stg
|colspan="1" style = "font-size:84%;"|Expression is expected, and typically strong
|-
|colspan="1" style = "font-size:90%; color:black;" |CD23
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression of CD23 is infrequent in HCL
|-
|colspan="1" style = "font-size:90%; color:black;" |CD38
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|wk
|colspan="1" style = "font-size:84%;"|Not typically expressed by HCL, may be seen
|-
|colspan="1" style = "font-size:90%;" |CD43
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Tends to be expressed at relatively high intensity
|-
|colspan="1" style = "font-size:90%; color:black;" |CD79b
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|wk
|colspan="1" style = "font-size:84%;"|Expression is expected
|-
|colspan="1" style = "font-size:90%;" |CD138
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Predominantly by cells with plasma cell differentiation
|-
|colspan="1" style = "font-size:90%; color:black;" |CD200
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Characteristically positive in HCL and often strong
|-
|}
</div>

'''Key to table: '''

Markers most useful in initial diagnosis are underlined in blue text

Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]

Key to expression strength code and use [[Expression strength key|Click for link]]

----

← Older revision		Revision as of 22:24, 4 July 2023
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	[[file:~~CLLCD45~~.jpg\|300px\|link=~~CLLCD45~~.jpg]]		[[file:HCLLCD45.jpg\|300px\|link=HCLCD45.jpg]]


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	\|colspan="1" style = "font-size:80%; width: 80%; \|'''Comment'''		\|colspan="1" style = "font-size:80%; width: 80%; \|'''Comment'''
	\|-		\|-
	\|colspan="1" style = "width: 4%; font-size:90%; color:black;" \|~~'''~~κ/λ~~'''~~		\|colspan="1" style = "width: 4%; font-size:90%; color:black;" \|κ/λ
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%; width: 8%; \|Stg		\|colspan="1" style = "font-size:80%; width: 8%; \|Stg
	\|colspan="1" style = "font-size:80%;"\|Expect strong restricted expression of κ or λ		\|colspan="1" style = "font-size:80%;"\|Expect strong restricted expression of κ or λ
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~CD19~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD19
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:84%;"\|Expression is expected and may be strong in HCL		\|colspan="1" style = "font-size:84%;"\|Expression is expected and may be strong in HCL
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|~~'''~~CD11C~~'''~~		\|colspan="1" style = "font-size:90%;" \|CD11C
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|Stg		\|colspan="1" style = "font-size:80%;"\|Stg
	\|colspan="1" style = "font-size:84%;"\|Strong expression of CD11c is typical of HCL		\|colspan="1" style = "font-size:84%;"\|Strong expression of CD11c is typical of HCL
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~CD25~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|CD25
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|wk		\|colspan="1" style = "font-size:80%;"\|wk
	\|colspan="1" style = "font-size:84%;"\|A typical and most frequently strongly expressed marker		\|colspan="1" style = "font-size:84%;"\|A typical and most frequently strongly expressed marker
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|~~'''~~FMC7~~'''~~		\|colspan="1" style = "font-size:90%; color:black;" \|FMC7
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|Stg		\|colspan="1" style = "font-size:80%;"\|Stg
	\|colspan="1" style = "font-size:80%;"\|Typically this marker is strongly expressed		\|colspan="1" style = "font-size:80%;"\|Typically this marker is strongly expressed
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|~~'''~~CD103~~'''~~		\|colspan="1" style = "font-size:90%;" \|CD103
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-