Flow cytometry: Diffuse Large B-cell Lymphoma - Revision history

John at 15:22, 13 July 2023

2023-07-13T15:22:09Z

← Older revision		Revision as of 17:22, 13 July 2023
Line 13:		Line 13:
	{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"		{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"
	!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of DLBCL'''		!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"\|'''Immunophenotype of DLBCL'''
			\|-
			!colspan="4" style = "font-size:90%; background:#DAE8F2"\|'''CD45''' expression
			\|-
			\|colspan="4" style = "font-size:90%; color:black;" \|Typically expression of CD45 approaches that of normal T and B cells, rarely expression is absent.
	\|-		\|-
	!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in CLL diagnosis		!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Major markers useful in CLL diagnosis

John at 08:07, 13 July 2023

2023-07-13T08:07:17Z

← Older revision		Revision as of 10:07, 13 July 2023
Line 6:		Line 6:
	<span style="color: navy>'''Background to flow cytometric diagnosis'''</span>		<span style="color: navy>'''Background to flow cytometric diagnosis'''</span>

	This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most ~~frequet penotypic~~ features, but emphasises that there is no absolutely typical pattern that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease so in many cases a diagnosis has already been made. Where these cases present as a new diagnosis by blood or marrow diagnosis requires careful correllation with other features.		This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequent phenotypic features, but emphasises that there is '''no absolutely typical pattern''' that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease so in many cases a diagnosis has already been made. Where these cases present as a new diagnosis by blood or marrow diagnosis requires careful correllation with other features.


Line 35:		Line 35:
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:80%;"\|Uncommon but seen in some cases		\|colspan="1" style = "font-size:80%;"\|Uncommon but seen in some cases
			\|-
			\|colspan="1" style = "font-size:90%;" \|CD10
			\|colspan="1" style = "background:#006699;"\|
			\|colspan="1" style = "font-size:80%;"\|-
			\|colspan="1" style = "font-size:84%;"\|Expression may be seen
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|CD79b		\|colspan="1" style = "font-size:90%; color:black;" \|CD79b
Line 52:		Line 57:
	\|-		\|-
	!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Other relevant markers		!colspan="4" style = "font-size:90%; background:#DAE8F2"\|Other relevant markers
	\|-
	~~\|colspan="1" style = "font-size:90%;" \|CD10~~
	~~\|colspan="1" style = "background:#006699;"\|~~
	~~\|colspan="1" style = "font-size:80%;"\|-~~
	~~\|colspan="1" style = "font-size:84%;"\|Expression may be seen~~
	\|-		\|-
	\|colspan="1" style = "font-size:90%;" \|CD11C		\|colspan="1" style = "font-size:90%;" \|CD11C

John at 08:05, 13 July 2023

2023-07-13T08:05:36Z

← Older revision		Revision as of 10:05, 13 July 2023
Line 35:		Line 35:
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:80%;"\|Uncommon but seen in some cases		\|colspan="1" style = "font-size:80%;"\|Uncommon but seen in some cases
	\|-
	~~\|colspan="1" style = "font-size:90%; color:black;" \|CD23~~
	~~\|colspan="1" style = "background:#00b8e6;"\|~~
	~~\|colspan="1" style = "font-size:80%;"\|-~~
	~~\|colspan="1" style = "font-size:84%;"\|Infrequent but reported~~
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|CD79b		\|colspan="1" style = "font-size:90%; color:black;" \|CD79b
Line 72:		Line 67:
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:84%;"\|Expect expression, rarely it is absent		\|colspan="1" style = "font-size:84%;"\|Expect expression, rarely it is absent
			\|-
			\|colspan="1" style = "font-size:90%; color:black;" \|CD23
			\|colspan="1" style = "background:#00b8e6;"\|
			\|colspan="1" style = "font-size:80%;"\|-
			\|colspan="1" style = "font-size:84%;"\|Infrequent but reported
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|CD25		\|colspan="1" style = "font-size:90%; color:black;" \|CD25

John at 08:04, 13 July 2023

2023-07-13T08:04:47Z

← Older revision		Revision as of 10:04, 13 July 2023
Line 76:		Line 76:
	\|colspan="1" style = "background:#66e0ff;"\|		\|colspan="1" style = "background:#66e0ff;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:84%;"\|		\|colspan="1" style = "font-size:84%;"\|Expressed in rare cases
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|CD38		\|colspan="1" style = "font-size:90%; color:black;" \|CD38

John at 08:03, 13 July 2023

2023-07-13T08:03:23Z

← Older revision		Revision as of 10:03, 13 July 2023
Line 29:		Line 29:
	\|colspan="1" style = "width: 8%; background:#006699;"\|		\|colspan="1" style = "width: 8%; background:#006699;"\|
	\|colspan="1" style = "font-size:80%; width: 8%; \|		\|colspan="1" style = "font-size:80%; width: 8%; \|
	\|colspan="1" style = "font-size:80%;"\|Light chain ~~retriction~~ usually detected		\|colspan="1" style = "font-size:80%;"\|Light chain restriction usually detected
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|CD5		\|colspan="1" style = "font-size:90%; color:black;" \|CD5
Line 71:		Line 71:
	\|colspan="1" style = "background:#004466;"\|		\|colspan="1" style = "background:#004466;"\|
	\|colspan="1" style = "font-size:80%;"\|-		\|colspan="1" style = "font-size:80%;"\|-
	\|colspan="1" style = "font-size:84%;"\|Expect expression		\|colspan="1" style = "font-size:84%;"\|Expect expression, rarely it is absent
	\|-		\|-
	\|colspan="1" style = "font-size:90%; color:black;" \|CD25		\|colspan="1" style = "font-size:90%; color:black;" \|CD25

John: Created page with "---- link=BLBCL.jpg
2023-07-12T12:21:50Z

Created page with "---- 200px|link=BLBCL.jpg '''Background to flow cytometric diagnosis''' This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequet penotypic features, but emphasises that there is no absolutely typical pattern that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only..."

New page
----

[[file:DLBCL.jpg|200px|link=BLBCL.jpg]]

'''Background to flow cytometric diagnosis'''

This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequet penotypic features, but emphasises that there is no absolutely typical pattern that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease so in many cases a diagnosis has already been made. Where these cases present as a new diagnosis by blood or marrow diagnosis requires careful correllation with other features.

<div style="width: 95%; overflow: auto; border: 1px solid navy; font-size:100%">
{| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5"
!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of DLBCL'''
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in CLL diagnosis
|-
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker'''
|colspan="1" style = "font-size:80%; width: 8%; |'''Freq'''
|colspan="1" style = "font-size:80%; width: 8%; |'''Level'''
|colspan="1" style = "font-size:80%; width: 80%; |'''Comment'''
|-
|colspan="1" style = "font-size:90%; color:black;" |CD19
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Generally CD19 will be expressed
|-
|colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ
|colspan="1" style = "width: 8%; background:#006699;"|
|colspan="1" style = "font-size:80%; width: 8%; |
|colspan="1" style = "font-size:80%;"|Light chain retriction usually detected
|-
|colspan="1" style = "font-size:90%; color:black;" |CD5
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:80%;"|Uncommon but seen in some cases
|-
|colspan="1" style = "font-size:90%; color:black;" |CD23
|colspan="1" style = "background:#00b8e6;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Infrequent but reported
|-
|colspan="1" style = "font-size:90%; color:black;" |CD79b
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Generally expressed
|-
|colspan="1" style = "font-size:90%; color:black;" |CD200
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Detected infrequently
|-
|colspan="1" style = "font-size:90%; color:black;" |FMC7
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|
|colspan="1" style = "font-size:80%;"|Generally expressed
|-
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers
|-
|colspan="1" style = "font-size:90%;" |CD10
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expression may be seen
|-
|colspan="1" style = "font-size:90%;" |CD11C
|colspan="1" style = "background:#006699;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%;" |CD20
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|Expect expression
|-
|colspan="1" style = "font-size:90%; color:black;" |CD25
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%; color:black;" |CD38
|colspan="1" style = "background:#004466;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%;" |CD43
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%;" |CD103
|colspan="1" style = "background:#E6FAFF;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|colspan="1" style = "font-size:90%;" |CD138
|colspan="1" style = "background:#66e0ff;"|
|colspan="1" style = "font-size:80%;"|-
|colspan="1" style = "font-size:84%;"|
|-
|}
</div>

'''Key to table: '''

Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]

Key to expression strength code and use [[Expression strength key|Click for link]]

----