Flow Cytometry:T-Lymphoid lineage assignment - Revision history

John at 18:22, 5 January 2024

2024-01-05T18:22:54Z

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	!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''~~Requirements to assign~~ T-lymphoid lineage'''</br></span>		!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''Assignment of T-lymphoid lineage'''</br></span>
	<span style="font-size:80%; text-align:left;"></br>For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br>
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	\|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" \|~~'''Marker requirement'''~~		\|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" \|For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br>
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	\|colspan="1" style = "font-size:90%; color:black;"\|'''Either''' [[CD3\|CD3 surface or cytoplasm (strong)]] '''Or''' [[CD3\|CD3 by immunocytochemistry (strong)]]		\|colspan="1" style = "font-size:90%; color:black;"\|'''Either''' [[CD3\|CD3 surface or cytoplasm (strong)]] '''Or''' [[CD3\|CD3 by immunocytochemistry (strong)]]

John at 18:21, 5 January 2024

2024-01-05T18:21:05Z

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	!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''Requirements to assign T-lymphoid lineage'''</br></span>		!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''Requirements to assign T-lymphoid lineage'''</br></span>
	~~<div class="mw-collapsible mw-collapsed" data-expandtext="Click for explanation" data-collapsetext="Hide explanation">~~
	~~<div class="mw-collapsible-content">~~
	<span style="font-size:80%; text-align:left;"></br>For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br>		<span style="font-size:80%; text-align:left;"></br>For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br>
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	\|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" \|'''Marker requirement'''		\|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" \|'''Marker requirement'''

John at 10:58, 5 January 2024

2024-01-05T10:58:53Z

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	\| colspan="1"''\|[[~~Flow cytometry:MPAL~~\|Return to previous page]]''		\| colspan="1"''\|[[Tables of diagnostic markers supporting lineage assignment in AML\|Return to previous page]]''
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John at 12:35, 22 December 2023

2023-12-22T12:35:17Z

← Older revision		Revision as of 14:35, 22 December 2023
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	----		----
	~~<span id="anchor_0">‎</span>.~~
	~~<div id="MPAL_Table3">'''Table 3'''</div>~~
	~~'''T-LYMPHOID LINEAGE ASSIGNMENT'''~~
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	!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''Requirements to assign T-lymphoid lineage ~~in MPAL~~'''</br></span>		!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''Requirements to assign T-lymphoid lineage'''</br></span>
	<div class="mw-collapsible mw-collapsed" data-expandtext="Click for explanation" data-collapsetext="Hide explanation">		<div class="mw-collapsible mw-collapsed" data-expandtext="Click for explanation" data-collapsetext="Hide explanation">
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John at 12:34, 22 December 2023

2023-12-22T12:34:49Z

← Older revision		Revision as of 14:34, 22 December 2023
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			----
			<span id="anchor_0">‎</span>.
			<div id="MPAL_Table3">'''Table 3'''</div>
			'''T-LYMPHOID LINEAGE ASSIGNMENT'''
			<div style="width: 100%; border: 1px solid black; font-size:100%">
			{\| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="0"
			!colspan="2" <span style="font-size:90%; font-color:navy; style="text-align: left; border: 1px solid black; background:pale gray">\|'''Requirements to assign T-lymphoid lineage in MPAL'''</br></span>
			<div class="mw-collapsible mw-collapsed" data-expandtext="Click for explanation" data-collapsetext="Hide explanation">
			<div class="mw-collapsible-content">
			<span style="font-size:80%; text-align:left;"></br>For T-lymphoid lineage assignment the key lineage-marker is CD3. Provided that this marker meets the intensity criteria then it is T-lineage defining in MPAL. This expression can be determined either by CD3 detection by flow cytometry OR by immunocytochemistry on trephine.</br>
			</span>
			</div></div></div>
			\|-
			\|colspan="2" style = "font-size:90%; color:black; background:#ddeee1" \|'''Marker requirement'''
			\|-
			\|colspan="1" style = "font-size:90%; color:black;"\|'''Either''' [[CD3\|CD3 surface or cytoplasm (strong)]] '''Or''' [[CD3\|CD3 by immunocytochemistry (strong)]]
			\|colspan="1" style = "font-size:90%;"\|To meet the definition of "strong" the expression intensity '''must exceed''' that of 50% of normal T lymphocytes
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			\|}
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			<span style="font-size:85%;>'''Notes on interpretation of T-lineage in MPAL'''</br>
			Expression of CD3 is required to assign T lineage in MPAL. Generally, other T-associated antigens will also be expressed:.CD7, and in the majority of cases. CD2, CD4, CD5, and CD8. Consider also that a proportion of early T precursor lymphoblastic leukaemia/lymphoma (ETP-ALL) may be difficult to distinguish from MPAL-T/M. Using an appropriate threshold for MPO positivity may be helpful and a threshold of >10% for myeloperoxidase positivity is broadly advised by WHO in this context.
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2023-12-22T12:33:29Z

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