BPDCN - Revision history

John at 11:59, 2 January 2024

2024-01-02T11:59:45Z

← Older revision		Revision as of 13:59, 2 January 2024
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	*BPCDN typically have features of primitive phenotype: the cells typically lie in the "blast area" - with dim to moderate CD45 expression with low side scatter. CD7, TdT, CD38 and HLA-DR are frequently expressed. '''CD34 is characteristically absent'''		*BPCDN typically have features of primitive phenotype: the cells typically lie in the "blast area" - with dim to moderate CD45 expression with low side scatter. CD7, TdT, CD38 and HLA-DR are frequently expressed. '''CD34 is characteristically absent'''
	*'''Lineage-defining markers of myeloid, T or B cell are not expressed''': i.e. there is no expression of MPO, CD14 or lysozyme; cCD3 is not expressed, and CD19 is not expressed (as well as CD20, cCD22, CD79a)		*'''Lineage-defining markers of myeloid, T or B cell are not expressed''': i.e. there is no expression of MPO, CD14 or lysozyme; cCD3 is not expressed, and CD19 is not expressed (as well as CD20, cCD22, CD79a)
	*'''One or more myeloid lineage-associated features may be detected''': expression of CD33 is relatively common (x%), CD117 may also be expressed (x%), CD13 may be found (x%). Therefore cases may fit the criteria to assign myeloid lineage.		*'''One or more myeloid lineage-associated features may be detected''': expression of CD33 is relatively common (>50%), CD117 may also be expressed (<20%), CD13 may be found although is infrequent (<5%). Therefore cases may fit the criteria to assign myeloid lineage (two myeloid-associated markers).
	</br>Diagnosis is then based on specific criteria:		</br>Diagnosis is then based on specific criteria:

John at 11:50, 2 January 2024

2024-01-02T11:50:48Z

← Older revision		Revision as of 13:50, 2 January 2024
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	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1
	\|-		\|-
	!colspan="2" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2)		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2)
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed
	\|}		\|}

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John at 11:50, 2 January 2024

2024-01-02T11:50:34Z

← Older revision		Revision as of 13:50, 2 January 2024
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	!colspan="2" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''		!colspan="2" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''
	\|-		\|-
	!colspan="2" style = "background:palegrey;border:solid"\|Then will also meet either Criteria set 1 or Criteria set 2</br>		!colspan="2" style = "background:palegrey;border:solid"\|Then will also meet either Criteria set 1 '''OR''' Criteria set 2</br>
	\|-		\|-
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 1)		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 1)
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1
	\|-		\|-
	!colspan="2" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2): '''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed		!colspan="2" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2)
			!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed
	\|}		\|}

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John at 11:49, 2 January 2024

2024-01-02T11:49:37Z

← Older revision		Revision as of 13:49, 2 January 2024
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	<div style="width: 95%"; >		<div style="width: 95%"; >
	{\| class="wikitable"		{\| class="wikitable"
	!colspan="1" style = "background:palegrey;border:solid"\|For cases meeting the criteria above, then the following are required:</br>		!colspan="2" style = "background:palegrey;border:solid"\|For cases meeting the criteria above, then the following are required:</br>
	\|-		\|-
	!colspan="1" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''		!colspan="2" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''
	\|-		\|-
	!colspan="1" style = "background:palegrey;border:solid"\|Then will also meet either Criteria set 1 or Criteria set 2</br>		!colspan="2" style = "background:palegrey;border:solid"\|Then will also meet either Criteria set 1 or Criteria set 2</br>
	\|-		\|-
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 1): '''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 1)
			!colspan="1" style = "background:white;border:solid; font-size:90%;"\|'''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1
	\|-		\|-
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2): '''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed		!colspan="2" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2): '''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed
	\|}		\|}

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John at 13:06, 31 December 2023

2023-12-31T13:06:46Z

← Older revision		Revision as of 15:06, 31 December 2023
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	This is a rare diagnosis but one that presents some practical diagnostic difficulties since BPDCN is derived from myeloid cells and therefore ~~shares~~ morphological and ~~some~~ immunophenotypic characteristics which ~~may~~ overlap typical AML. Morphologically the primitive cells may resemble AML or have lymphoid morphology, ~~with~~ a skin rash present in most cases. ~~'''NOTE''' other hematologic malignancies may share similar phenotypes with BPDCN~~, ~~therefore diagnosis requires~~ correlation with morphology, histopathology and clinical information.</br></br>		This is a rare diagnosis but one that presents some practical diagnostic difficulties since BPDCN is derived from myeloid cells and therefore may have morphological and immunophenotypic characteristics which overlap with typical AML or acute undifferentiated leukaemia. Morphologically the primitive cells may resemble AML or have lymphoid morphology, and a skin rash is present in most cases. In view of the diagnostic overlap, correlation with morphology, histopathology and clinical information is important when making this diagnosis.</br></br>

	Using standard diagnostic panels, cases of BPDCN generally have the following features:		Using standard diagnostic panels, cases of BPDCN generally have the following features:

John at 18:48, 29 December 2023

2023-12-29T18:48:46Z

← Older revision		Revision as of 20:48, 29 December 2023
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	!colspan="1" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''		!colspan="1" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''
	\|-		\|-
	!colspan="1" style = "background:palegrey;border:solid"\|Then will also meet Criteria 1</br>		!colspan="1" style = "background:palegrey;border:solid"\|Then will also meet either Criteria set 1 or Criteria set 2</br>
	\|-		\|-
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (1): '''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 1): '''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1
	\|-		\|-
	~~!colspan="1" style = "background:palegrey;border:solid"\|Or will meet Criteria 2</br>~~		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (set 2): '''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed
	\|-
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (2): '''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed
	\|}		\|}

	----		----

John at 18:47, 29 December 2023

2023-12-29T18:47:11Z

← Older revision		Revision as of 20:47, 29 December 2023
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	<div style="width: 95%"; >		<div style="width: 95%"; >
	{\| class="wikitable"		{\| class="wikitable"
	!colspan="1" style = "background:palegrey;border:solid"\|~~Initial Criteria~~</br>		!colspan="1" style = "background:palegrey;border:solid"\|For cases meeting the criteria above, then the following are required:</br>
	\|-		\|-
	!colspan="1" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: bright CD56 and/or CD4		!colspan="1" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: '''bright CD56 and/or CD4'''
	\|-		\|-
	!colspan="1" style = "background:palegrey;border:solid"\|~~The~~ will ~~express either~~ Criteria 1</br>		!colspan="1" style = "background:palegrey;border:solid"\|Then will also meet Criteria 1</br>
	\|-		\|-
	!colspan="1" style = "background:white;border:solid; font-size:90%;"\|~~BPDCN associate markers~~: '''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1		!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (1): '''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1
			\|-
			!colspan="1" style = "background:palegrey;border:solid"\|Or will meet Criteria 2</br>
			\|-
			!colspan="1" style = "background:white;border:solid; font-size:90%;"\|Criteria for BPCN (2): '''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed
	\|}		\|}

	~~'''Diagnostic criteria for BPDCN:'''~~

	~~Should express: bright CD56 and/or CD4~~

	~~then~~

	~~BPDCN associate markers:~~

	~~'''CD123 expressed''' and one of: CD303, CD304, TCF4, TCL1~~

	Or

	~~'''CD123 not expressed''' but at least three of CD303, CD304, TCF4, TCL1 are expressed and context of negative markers (above) is met~~

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John at 18:42, 29 December 2023

2023-12-29T18:42:59Z

← Older revision		Revision as of 20:42, 29 December 2023
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	</br>Diagnosis is then based on specific criteria:		</br>Diagnosis is then based on specific criteria:

			<div style="width: 95%"; >
			{\| class="wikitable"
			!colspan="1" style = "background:palegrey;border:solid"\|Initial Criteria</br>
			\|-
			!colspan="1" style = "background:white; font-size:90%; border:solid; "\|Cells of BPDCN will express: bright CD56 and/or CD4
			\|-
			!colspan="1" style = "background:palegrey;border:solid"\|The will express either Criteria 1</br>
			\|-
			!colspan="1" style = "background:white;border:solid; font-size:90%;"\|BPDCN associate markers: '''CD123 expressed''' together with one of: CD303, CD304, TCF4, TCL1
			\|}

	'''Diagnostic criteria for BPDCN:'''		'''Diagnostic criteria for BPDCN:'''

John at 18:39, 29 December 2023

2023-12-29T18:39:09Z

← Older revision		Revision as of 20:39, 29 December 2023
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	----		----

	'''Blastic plasmacytoid dendritic cell neoplasm (BPDCN)'''		<div style="width: 95%">
			{\| class="wikitable" style="border-style: solid; border-width: 5px; color:black"
			\|colspan="1" style = "font-size:90%; color:black; background:#ddeee1"\|'''Blastic plasmacytoid dendritic cell neoplasm (BPDCN)'''
			\|}

John at 18:10, 29 December 2023

2023-12-29T18:10:47Z

← Older revision		Revision as of 20:10, 29 December 2023
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	*'''Lineage-defining markers of myeloid, T or B cell are not expressed''': i.e. there is no expression of MPO, CD14 or lysozyme; cCD3 is not expressed, and CD19 is not expressed (as well as CD20, cCD22, CD79a)		*'''Lineage-defining markers of myeloid, T or B cell are not expressed''': i.e. there is no expression of MPO, CD14 or lysozyme; cCD3 is not expressed, and CD19 is not expressed (as well as CD20, cCD22, CD79a)
	*'''One or more myeloid lineage-associated features may be detected''': expression of CD33 is relatively common (x%), CD117 may also be expressed (x%), CD13 may be found (x%). Therefore cases may fit the criteria to assign myeloid lineage.		*'''One or more myeloid lineage-associated features may be detected''': expression of CD33 is relatively common (x%), CD117 may also be expressed (x%), CD13 may be found (x%). Therefore cases may fit the criteria to assign myeloid lineage.
	*Diagnosis is based on specific criteria ~~(below)~~:		</br>Diagnosis is then based on specific criteria:


	~~----~~

	'''Diagnostic criteria for BPDCN:'''		'''Diagnostic criteria for BPDCN:'''