Flow cytometry: Mantle cell Lymphoma: Difference between revisions
From haematologyetc.co.uk
No edit summary |
No edit summary |
||
(2 intermediate revisions by the same user not shown) | |||
Line 2: | Line 2: | ||
[[file:MCL.jpg|200px|link=MCL.jpg]] | [[file:MCL.jpg|200px|link=MCL.jpg]] | ||
Line 11: | Line 9: | ||
Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200. | Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200. | ||
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm. | However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of CLL or MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm. | ||
Latest revision as of 16:18, 11 July 2023
Background
Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200.
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of CLL or MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm.
Immunophenotype of MCL | |||
---|---|---|---|
Major markers useful in diagnosis | |||
Marker | Freq | Level | Comment |
κ/λ | Expect moderate or strong restricted κ or λ expression | ||
CD19 | - | Expression is expected, typically more strongly than in CLL | |
CD5 | - | Expression of CD5 is characteristic of MCL | |
CD23 | - | Typically not expressed by MCL, may rarely be expressed | |
CD79b | Expression is expected and should be stronger than CLL | ||
CD200 | - | Characteristically (though not always) negative in MCL | |
FMC7 | - | FMC7 is frequently expressed by typical MCL | |
Other relevant markers | |||
CD10 | - | Infrequently expressed, expression may suggest FL | |
CD11C | Expressed in some cases of MCL but not typical | ||
CD20 | - | Expression is expected, typically stronger than CLL | |
CD25 | Not typical of MCL, not generally strong as HCL | ||
CD38 | - | Expression is expected in MCL but CD138 should be absent | |
CD43 | - | Lower expression than in CLL, may be helpful in some cases | |
CD103 | - | Not expressed by MCL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link