Flow cytometry: Mantle cell Lymphoma: Difference between revisions
From haematologyetc.co.uk
No edit summary |
No edit summary |
||
(14 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
---- | |||
[[file:MCL.jpg|200px|link=MCL.jpg]] | |||
Line 5: | Line 9: | ||
Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200. | Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200. | ||
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm. | However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of CLL or MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm. | ||
Line 13: | Line 17: | ||
!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of MCL''' | !colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of MCL''' | ||
|- | |- | ||
!colspan="4" style = "font-size:90%; background:#DAE8F2"| | !colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in diagnosis | ||
|- | |- | ||
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker''' | |colspan="1" style = "font-size:80%; width: 4%; |'''Marker''' | ||
Line 20: | Line 24: | ||
|colspan="1" style = "font-size:80%; width: 80%; |'''Comment''' | |colspan="1" style = "font-size:80%; width: 80%; |'''Comment''' | ||
|- | |- | ||
|colspan="1" style = "width: 4%; font-size:90%; color: | |colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ | ||
|colspan="1" style = "width: 8%; background:#004466;"| | |colspan="1" style = "width: 8%; background:#004466;"| | ||
|colspan="1" style = "font-size:80%; width: 8%; | | |colspan="1" style = "font-size:80%; width: 8%; | | ||
|colspan="1" style = "font-size:80%;"|Expect moderate or strong restricted κ or λ expression | |colspan="1" style = "font-size:80%;"|Expect moderate or strong restricted κ or λ expression | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color: | |colspan="1" style = "font-size:90%; color:black;" |CD19 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#004466;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size: | |colspan="1" style = "font-size:84%;"|Expression is expected, typically more strongly than in CLL | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color: | |colspan="1" style = "font-size:90%; color:black;" |CD5 | ||
|colspan="1" style = "background:#004466;"| | |colspan="1" style = "background:#004466;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:80%;"|Expression of CD5 is characteristic of MCL | |colspan="1" style = "font-size:80%;"|Expression of CD5 is characteristic of MCL | ||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |CD23 | |||
|colspan="1" style = "background:#00b8e6;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:84%;"|Typically not expressed by MCL, may rarely be expressed | |||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |CD79b | |||
|colspan="1" style = "background:#004466;"| | |||
|colspan="1" style = "font-size:80%;"| | |||
|colspan="1" style = "font-size:84%;"|Expression is expected and should be stronger than CLL | |||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |CD200 | |||
|colspan="1" style = "background:#E6FAFF;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:84%;"|Characteristically (though not always) negative in MCL | |||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |FMC7 | |||
|colspan="1" style = "background:#006699;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:80%;"|FMC7 is frequently expressed by typical MCL | |||
|- | |||
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers | |||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD10 | |colspan="1" style = "font-size:90%;" |CD10 | ||
Line 44: | Line 70: | ||
|colspan="1" style = "font-size:80%;"| | |colspan="1" style = "font-size:80%;"| | ||
|colspan="1" style = "font-size:84%;"|Expressed in some cases of MCL but not typical | |colspan="1" style = "font-size:84%;"|Expressed in some cases of MCL but not typical | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD20 | |colspan="1" style = "font-size:90%;" |CD20 | ||
Line 54: | Line 75: | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Expression is expected, typically stronger than CLL | |colspan="1" style = "font-size:84%;"|Expression is expected, typically stronger than CLL | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color:black;" |CD25 | |colspan="1" style = "font-size:90%; color:black;" |CD25 | ||
Line 74: | Line 90: | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Lower expression than in CLL, may be helpful in some cases | |colspan="1" style = "font-size:84%;"|Lower expression than in CLL, may be helpful in some cases | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD103 | |colspan="1" style = "font-size:90%;" |CD103 | ||
Line 89: | Line 100: | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation | |colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation | ||
|- | |- | ||
|} | |} | ||
Line 101: | Line 107: | ||
<span style="color:navy;font-size:80% ">'''Key to table: '''</span> | <span style="color:navy;font-size:80% ">'''Key to table: '''</span> | ||
<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span> | <span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span> |
Latest revision as of 16:18, 11 July 2023
Background
Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200.
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of CLL or MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm.
Immunophenotype of MCL | |||
---|---|---|---|
Major markers useful in diagnosis | |||
Marker | Freq | Level | Comment |
κ/λ | Expect moderate or strong restricted κ or λ expression | ||
CD19 | - | Expression is expected, typically more strongly than in CLL | |
CD5 | - | Expression of CD5 is characteristic of MCL | |
CD23 | - | Typically not expressed by MCL, may rarely be expressed | |
CD79b | Expression is expected and should be stronger than CLL | ||
CD200 | - | Characteristically (though not always) negative in MCL | |
FMC7 | - | FMC7 is frequently expressed by typical MCL | |
Other relevant markers | |||
CD10 | - | Infrequently expressed, expression may suggest FL | |
CD11C | Expressed in some cases of MCL but not typical | ||
CD20 | - | Expression is expected, typically stronger than CLL | |
CD25 | Not typical of MCL, not generally strong as HCL | ||
CD38 | - | Expression is expected in MCL but CD138 should be absent | |
CD43 | - | Lower expression than in CLL, may be helpful in some cases | |
CD103 | - | Not expressed by MCL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link