Flow cytometry: Mantle Cell Lymphoma: Difference between revisions
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(Created page with "---- 200px|link=MCL.jpg <span style="color: navy>'''Background'''</span> Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200. However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of CLL or MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confi...") |
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!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of MCL''' | !colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of MCL''' | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | |||
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|colspan="4" style = "font-size:90%; color:black;" |Typically expression of CD45 is weaker than in normal T or B cells although stronger and less variable than in typical CLL. | |||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in diagnosis | !colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in diagnosis | ||
Latest revision as of 17:16, 13 July 2023
Background
Typical MCL expresses CD5, but can be distinguished from CLL by the absence of CD23 and CD200.
However, individual markers may not be expressed in all cases - this can cause diagnostic difficulty particularly with atypical forms of CLL or MZL or occasionally other B-cell lymphoproliferative disorders. Generally it is correct to advise cytogenetic or genetic testing to confirm.
| Immunophenotype of MCL | |||
|---|---|---|---|
| CD45 expression | |||
| Typically expression of CD45 is weaker than in normal T or B cells although stronger and less variable than in typical CLL. | |||
| Major markers useful in diagnosis | |||
| Marker | Freq | Level | Comment |
| κ/λ | Expect moderate or strong restricted κ or λ expression | ||
| CD19 | - | Expression is expected, typically more strongly than in CLL | |
| CD5 | - | Expression of CD5 is characteristic of MCL | |
| CD23 | - | Typically not expressed by MCL, may rarely be expressed | |
| CD79b | Expression is expected and should be stronger than CLL | ||
| CD200 | - | Characteristically (though not always) negative in MCL | |
| FMC7 | - | FMC7 is frequently expressed by typical MCL | |
| Other relevant markers | |||
| CD10 | - | Infrequently expressed, expression may suggest FL | |
| CD11C | Expressed in some cases of MCL but not typical | ||
| CD20 | - | Expression is expected, typically stronger than CLL | |
| CD25 | Not typical of MCL, not generally strong as HCL | ||
| CD38 | - | Expression is expected in MCL but CD138 should be absent | |
| CD43 | - | Lower expression than in CLL, may be helpful in some cases | |
| CD103 | - | Not expressed by MCL, consider HCL if found | |
| CD138 | - | Predominantly expressed at plasma cell differentiation | |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link