Flow cytometry: Follicular Lymphoma: Difference between revisions
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file:FL.jpg|link={{filepath:FL.jpg}} | |||
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<span style="color: navy>'''Background'''</span> | <span style="color: navy>'''Background'''</span> | ||
Follicular lymphoma expresses CD10, with other immunophenotypic featues differing from related disorders. | |||
However, individual markers may not be expressed in all cases - | However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing. | ||
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!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of FL''' | !colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of FL''' | ||
|- | |- | ||
!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''Major markers useful in | !colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | ||
|- | |||
|colspan="4" style = "font-size:90%; color:black;" |Typically expression of CD45 approaches that of normal T and B cells. | |||
|- | |||
!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''Major markers useful in diagnosis''' | |||
|- | |- | ||
|colspan="1" style = "font-size:80%; width: 4%; |'''Marker''' | |colspan="1" style = "font-size:80%; width: 4%; |'''Marker''' | ||
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|colspan="1" style = "font-size:80%; width: 80%; |'''Comment''' | |colspan="1" style = "font-size:80%; width: 80%; |'''Comment''' | ||
|- | |- | ||
|colspan="1" style = "width: 4%; font-size:90%; color: | |colspan="1" style = "width: 4%; font-size:90%; color:black;" |κ/λ | ||
|colspan="1" style = "width: 8%; background:#006699;"| | |colspan="1" style = "width: 8%; background:#006699;"| | ||
|colspan="1" style = "font-size:80%; width: 8%; | | |colspan="1" style = "font-size:80%; width: 8%; | | ||
|colspan="1" style = "font-size:80%;"|Expect κ or λ expression, undetectable in rare cases | |colspan="1" style = "font-size:80%;"|Expect κ or λ expression, undetectable in rare cases | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color: | |colspan="1" style = "font-size:90%; color:black;" |CD19 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size: | |colspan="1" style = "font-size:84%;"|Expression is expected, but may be weak or undectable | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color: | |colspan="1" style = "font-size:90%; color:black;" |CD5 | ||
|colspan="1" style = "background:#E6FAFF;"| | |colspan="1" style = "background:#E6FAFF;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:80%;"|Expression of CD5 is characteristic of MCL | |colspan="1" style = "font-size:80%;"|Expression of CD5 is characteristic of MCL | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" | | |colspan="1" style = "font-size:90%; color:black;" |CD10 | ||
|colspan="1" style = "background:#004466;"| | |colspan="1" style = "background:#004466;"| | ||
|colspan="1" style = "font-size:80%;"|Strg | |colspan="1" style = "font-size:80%;"|Strg | ||
|colspan="1" style = "font-size:84%;"|Expression is expected though not universal in FL | |colspan="1" style = "font-size:84%;"|Expression is expected though not universal in FL | ||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |CD23 | |||
|colspan="1" style = "background:#00b8e6;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:84%;"|May be expressed by some cases of FL | |||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |CD79b | |||
|colspan="1" style = "background:#004466;"| | |||
|colspan="1" style = "font-size:80%;"| | |||
|colspan="1" style = "font-size:84%;"|Expression is expected and should be stronger than CLL | |||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |CD200 | |||
|colspan="1" style = "background:#E6FAFF;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:84%;"|Characteristically (though not always) negative in FL | |||
|- | |||
|colspan="1" style = "font-size:90%; color:black;" |FMC7 | |||
|colspan="1" style = "background:#00b8e6;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:80%;"|Occasionally seen, FMC7 is not typical of FL | |||
|- | |||
!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers | |||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD11C | |colspan="1" style = "font-size:90%;" |CD11C | ||
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|colspan="1" style = "font-size:80%;"| | |colspan="1" style = "font-size:80%;"| | ||
|colspan="1" style = "font-size:84%;"|Expression is not typically seen in FL | |colspan="1" style = "font-size:84%;"|Expression is not typically seen in FL | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD20 | |colspan="1" style = "font-size:90%;" |CD20 | ||
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|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Expression is expected, rare cases may have CD20 loss | |colspan="1" style = "font-size:84%;"|Expression is expected, rare cases may have CD20 loss | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color:black;" |CD25 | |colspan="1" style = "font-size:90%; color:black;" |CD25 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#E6FAFF;"| | ||
|colspan="1" style = "font-size:80%;"| | |colspan="1" style = "font-size:80%;"| | ||
|colspan="1" style = "font-size:84%;"|Not typical of | |colspan="1" style = "font-size:84%;"|Not typical of FL, where present it is not generally strong | ||
|- | |- | ||
|colspan="1" style = "font-size:90%; color:black;" |CD38 | |colspan="1" style = "font-size:90%; color:black;" |CD38 | ||
|colspan="1" style = "background:#004466;"| | |colspan="1" style = "background:#004466;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Expression is expected in | |colspan="1" style = "font-size:84%;"|Expression is expected in FL but CD138 should be absent | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD43 | |colspan="1" style = "font-size:90%;" |CD43 | ||
|colspan="1" style = "background:#00b8e6;"| | |colspan="1" style = "background:#00b8e6;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|Expression is uncommon in FL, may be helpful in some cases | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD103 | |colspan="1" style = "font-size:90%;" |CD103 | ||
|colspan="1" style = "background:#E6FAFF;"| | |colspan="1" style = "background:#E6FAFF;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Not expressed by | |colspan="1" style = "font-size:84%;"|Not expressed by FL, consider HCL if found | ||
|- | |- | ||
|colspan="1" style = "font-size:90%;" |CD138 | |colspan="1" style = "font-size:90%;" |CD138 | ||
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|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation | |colspan="1" style = "font-size:84%;"|Predominantly expressed at plasma cell differentiation | ||
|- | |- | ||
|} | |} | ||
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<span style="color:navy;font-size:80% ">'''Key to table: '''</span> | <span style="color:navy;font-size:80% ">'''Key to table: '''</span> | ||
<span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span> | <span style="font-size:80% ">Key to colour code for expression frequency [[Key_to_marker_expression_table|Click for link]]</span> |
Latest revision as of 17:17, 13 July 2023
Background
Follicular lymphoma expresses CD10, with other immunophenotypic featues differing from related disorders.
However, individual markers may not be expressed in all cases - confirmation can be made from histopathology, cytogenetic or genetic testing.
Immunophenotype of FL | |||
---|---|---|---|
CD45 expression | |||
Typically expression of CD45 approaches that of normal T and B cells. | |||
Major markers useful in diagnosis | |||
Marker | Freq | Level | Comment |
κ/λ | Expect κ or λ expression, undetectable in rare cases | ||
CD19 | - | Expression is expected, but may be weak or undectable | |
CD5 | - | Expression of CD5 is characteristic of MCL | |
CD10 | Strg | Expression is expected though not universal in FL | |
CD23 | - | May be expressed by some cases of FL | |
CD79b | Expression is expected and should be stronger than CLL | ||
CD200 | - | Characteristically (though not always) negative in FL | |
FMC7 | - | Occasionally seen, FMC7 is not typical of FL | |
Other relevant markers | |||
CD11C | Expression is not typically seen in FL | ||
CD20 | - | Expression is expected, rare cases may have CD20 loss | |
CD25 | Not typical of FL, where present it is not generally strong | ||
CD38 | - | Expression is expected in FL but CD138 should be absent | |
CD43 | - | Expression is uncommon in FL, may be helpful in some cases | |
CD103 | - | Not expressed by FL, consider HCL if found | |
CD138 | - | Predominantly expressed at plasma cell differentiation |
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link