Flow cytometry: Diffuse Large B-cell Lymphoma: Difference between revisions
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<span style="color: navy>'''Background to flow cytometric diagnosis'''</span> | <span style="color: navy>'''Background to flow cytometric diagnosis'''</span> | ||
This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most | This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequent phenotypic features, but emphasises that there is '''no absolutely typical pattern''' that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease so in many cases a diagnosis has already been made. Where these cases present as a new diagnosis by blood or marrow diagnosis requires careful correllation with other features. | ||
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{| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5" | {| class="wikitable" style="color:black; background-color:#ffffff;" cellpadding="5" | ||
!colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of DLBCL''' | !colspan="4" style = "font-size:110%; color: navy; text-align:center; background:#DEE0DE;"|'''Immunophenotype of DLBCL''' | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|'''CD45''' expression | |||
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|colspan="4" style = "font-size:90%; color:black;" |Typically expression of CD45 approaches that of normal T and B cells, rarely expression is absent. | |||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in CLL diagnosis | !colspan="4" style = "font-size:90%; background:#DAE8F2"|Major markers useful in CLL diagnosis | ||
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|colspan="1" style = "font-size:80%;"|Uncommon but seen in some cases | |colspan="1" style = "font-size:80%;"|Uncommon but seen in some cases | ||
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|colspan="1" style = "font-size:90% | |colspan="1" style = "font-size:90%;" |CD10 | ||
|colspan="1" style = "background:# | |colspan="1" style = "background:#006699;"| | ||
|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"| | |colspan="1" style = "font-size:84%;"|Expression may be seen | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD79b | |colspan="1" style = "font-size:90%; color:black;" |CD79b | ||
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!colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers | !colspan="4" style = "font-size:90%; background:#DAE8F2"|Other relevant markers | ||
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|colspan="1" style = "font-size:90%;" |CD11C | |colspan="1" style = "font-size:90%;" |CD11C | ||
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|colspan="1" style = "font-size:80%;"|- | |colspan="1" style = "font-size:80%;"|- | ||
|colspan="1" style = "font-size:84%;"|Expect expression, rarely it is absent | |colspan="1" style = "font-size:84%;"|Expect expression, rarely it is absent | ||
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|colspan="1" style = "font-size:90%; color:black;" |CD23 | |||
|colspan="1" style = "background:#00b8e6;"| | |||
|colspan="1" style = "font-size:80%;"|- | |||
|colspan="1" style = "font-size:84%;"|Infrequent but reported | |||
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|colspan="1" style = "font-size:90%; color:black;" |CD25 | |colspan="1" style = "font-size:90%; color:black;" |CD25 | ||
Latest revision as of 17:22, 13 July 2023
Background to flow cytometric diagnosis
This is an uncommon diagnosis by flow cytometry and the immunophenotype is less frequently reported. This table represents the most frequent phenotypic features, but emphasises that there is no absolutely typical pattern that sets this diagnosis apart from other B-LPD. However, DLBCL cells generally circulate and involve bone marrow only at only a late stage in disease so in many cases a diagnosis has already been made. Where these cases present as a new diagnosis by blood or marrow diagnosis requires careful correllation with other features.
| Immunophenotype of DLBCL | |||
|---|---|---|---|
| CD45 expression | |||
| Typically expression of CD45 approaches that of normal T and B cells, rarely expression is absent. | |||
| Major markers useful in CLL diagnosis | |||
| Marker | Freq | Level | Comment |
| CD19 | - | Generally CD19 will be expressed | |
| κ/λ | Light chain restriction usually detected | ||
| CD5 | - | Uncommon but seen in some cases | |
| CD10 | - | Expression may be seen | |
| CD79b | - | Generally expressed | |
| CD200 | - | Detected infrequently | |
| FMC7 | Generally expressed | ||
| Other relevant markers | |||
| CD11C | - | ||
| CD20 | - | Expect expression, rarely it is absent | |
| CD23 | - | Infrequent but reported | |
| CD25 | - | Expressed in rare cases | |
| CD38 | - | ||
| CD43 | - | ||
| CD103 | - | ||
| CD138 | - | ||
Key to table:
Key to colour code for expression frequency Click for link
Key to expression strength code and use Click for link